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A high-throughput assay for the comprehensive profiling of DNA ligase fidelity

Gregory J. S. Lohman, Robert J. Bauer, Nicole M. Nichols, Laurie Mazzola, Joanna Bybee, Danielle Rivizzigno, Elizabeth Cantin and Thomas C. Evans.

in Nucleic Acids Research

January 2016; p ublished online September 2015 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 8044 words.

DNA ligases have broad application in molecular biology, from traditional cloning methods to modern synthetic biology and molecular diagnostics protocols. Ligation-based detection of...

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A high-throughput assay for Tn<i>5</i> Tnp-induced DNA cleavage

Brandon Ason and William S. Reznikoff.

in Nucleic Acids Research

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Enzymology; Molecular and Cell Biology; Molecular Biology and Genetics. 3431 words.

Transposition causes genomic instability by mobilizing DNA elements. This phenomenon is mechanistically related to other DNA rearrangements, such as V(D)J recombination and retroviral DNA...

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Identification of human telomerase assembly inhibitors enabled by a novel method to produce hTERT

Guillaume Kellermann, Markus Kaiser, Florent Dingli, Olivier Lahuna, Delphine Naud-Martin, Florence Mahuteau-Betzer, Damarys Loew, Evelyne Ségal-Bendirdjian, Marie-Paule Teulade-Fichou and Sophie Bombard.

in Nucleic Acids Research

September 2015; p ublished online May 2015 .

Journal Article. Subjects: Proteins; Enzymology; Molecular and Cell Biology; Molecular Biology and Genetics. 7197 words.

Telomerase is the enzyme that maintains the length of telomeres. It is minimally constituted of two components: a core reverse transcriptase protein (hTERT) and an RNA (hTR). Despite its...

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<i>In vitro</i> selection of restriction endonucleases by <i>in vitro</i> compartmentalization

Nobuhide Doi, Shin Kumadaki, Yuko Oishi, Nobutaka Matsumura and Hiroshi Yanagawa.

in Nucleic Acids Research

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 4368 words.

Restriction endonucleases are widely used in laboratory applications from recombinant DNA technology to diagnostics, but engineering of restriction enzymes by structure-guided design and in...

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Ligase‐mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase

Maritha Mendel‐Hartvig, Anil Kumar and Ulf Landegren.

in Nucleic Acids Research

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 3641 words.

Branched nucleic acid strands exist as intermediates in certain biological reactions, and bifurcating DNA also presents interesting opportunities in biotechnological applications. We...

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Massively parallel determination and modeling of endonuclease substrate specificity

Summer B. Thyme, Yifan Song, T. J. Brunette, Mindy D. Szeto, Lara Kusak, Philip Bradley and David Baker.

in Nucleic Acids Research

December 2014; p ublished online November 2014 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics; Proteins; Research Methods in Life Sciences. 8116 words.

We describe the identification and characterization of novel homing endonucleases using genome database mining to identify putative target sites, followed by high throughput activity...

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Molecular events during translocation and proofreading extracted from 200 static structures of DNA polymerase

Zhong Ren.

in Nucleic Acids Research

September 2016; p ublished online June 2016 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics; Bioinformatics and Computational Biology. 12316 words.

DNA polymerases in family B are workhorses of DNA replication that carry out the bulk of the job at a high speed with high accuracy. A polymerase in this family relies on a built-in...

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A novel high-throughput activity assay for the <i>Trypanosoma brucei</i> editosome enzyme REL1 and other RNA ligases

Stephan Zimmermann, Laurence Hall, Sean Riley, Jesper Sørensen, Rommie E. Amaro and Achim Schnaufer.

in Nucleic Acids Research

February 2016; p ublished online September 2015 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics; Research Methods in Life Sciences. 7890 words.

The protist parasite Trypanosoma brucei causes Human African trypanosomiasis (HAT), which threatens millions of people in sub-Saharan Africa. Without treatment the infection is almost...

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Polyadenylation-dependent screening assay for respiratory syncytial virus RNA transcriptase activity and identification of an inhibitor

Stephen W. Mason, Carol Lawetz, Yvon Gaudette, Florence Dô, Erika Scouten, Lisette Lagacé, Bruno Simoneau and Michel Liuzzi.

in Nucleic Acids Research

September 2004; p ublished online September 2004 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 6964 words.

RNA-dependent RNA polymerase from respiratory syncytial virus (RSV) is a multi-subunit ribonucleoprotein (RNP) complex that, in addition to synthesizing the full 15 222 nt viral genomic RNA,...

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Quantitative telomerase enzyme activity determination using droplet digital PCR with single cell resolution

Andrew T. Ludlow, Jerome D. Robin, Mohammed Sayed, Claudia M. Litterst, Dawne N. Shelton, Jerry W. Shay and Woodring E. Wright.

in Nucleic Acids Research

July 2014; p ublished online May 2014 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 7312 words.

The telomere repeat amplification protocol (TRAP) for the human reverse transcriptase, telomerase, is a PCR-based assay developed two decades ago and is still used for routine determination...

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