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Real-time detection and continuous monitoring of ER stress <i>in vitro</i> and <i>in vivo</i> by ES-TRAP: evidence for systemic, transient ER stress during endotoxemia

Nobuhiko Hiramatsu, Ayumi Kasai, Kunihiro Hayakawa, Jian Yao and Masanori Kitamura.

in Nucleic Acids Research

August 2006; p ublished online August 2006 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics; Biological Sciences. 7489 words.

Activity of secreted alkaline phosphatase (SEAP) produced by transfected cells is rapidly down-regulated by endoplasmic reticulum (ER) stress independent of transcriptional regulation. This...

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A real-time fluorescence polarization activity assay to screen for inhibitors of bacterial ribonuclease P

Xin Liu, Yu Chen and Carol A. Fierke.

in Nucleic Acids Research

November 2014; p ublished online September 2014 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 9005 words.

Ribonuclease P (RNase P) is an essential endonuclease that catalyzes the 5′ end maturation of precursor tRNA (pre-tRNA). Bacterial RNase P is an attractive potential antibacterial target...

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Real-time investigation of nucleic acids phosphorylation process using molecular beacons

Zhiwen Tang, Kemin Wang, Weihong Tan, Changbei Ma, Jun Li, Lingfeng Liu, Qiuping Guo and Xiangxian Meng.

in Nucleic Acids Research

June 2005; p ublished online June 2005 .

Journal Article. Subjects: Research Methods in Life Sciences; Enzymology; Molecular Biology and Genetics. 4199 words.

Phosphorylation of nucleic acids is an indispensable process to repair strand interruption of nucleic acids. We have studied the process of phosphorylation using molecular beacon (MB) DNA...

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Real-time monitoring of enzymatic DNA hydrolysis by electrospray ionization mass spectrometry

Robert H. H. van den Heuvel, Sara Gato, Cees Versluis, Pascal Gerbaux, Colin Kleanthous and Albert J. R. Heck.

in Nucleic Acids Research

June 2005; p ublished online June 2005 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 5195 words.

A fast and direct method for the monitoring of enzymatic DNA hydrolysis was developed using electrospray ionization mass spectrometry. We incorporated the use of a robotic chip-based...

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Surface plasmon field-enhanced fluorescence spectroscopy studies of primer extension reactions

Gudrun Stengel and Wolfgang Knoll.

in Nucleic Acids Research

January 2005; p ublished online January 2005 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 7756 words.

Surface plasmon field-enhanced fluorescence spectroscopy (SPFS) utilizes the evanescent electromagnetic field of a surface plasmon to excite chromophors in close proximity to the surface....

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Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system

Natalia Morozova, Anton Sabantsev, Ekaterina Bogdanova, Yana Fedorova, Anna Maikova, Alexey Vedyaykin, Andjela Rodic, Marko Djordjevic, Mikhail Khodorkovskii and Konstantin Severinov.

in Nucleic Acids Research

January 2016; p ublished online December 2015 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 7370 words.

Type II restriction-modification (R-M) systems encode a restriction endonuclease that cleaves DNA at specific sites, and a methyltransferase that modifies same sites protecting them from...

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Use of a restriction enzyme-digested PCR product as substrate for helicase assays

Jae-Ho Shin, John N. Reeve and Zvi Kelman.

in Nucleic Acids Research

January 2005; p ublished online January 2005 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 2461 words.

DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise...

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