Oxford Index Browse

You are looking at 1-10 of 26 items for:

Science and Mathematics x Biochemistry x Enzymology x clear all

Adapting capillary gel electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes

Lucia Greenough, Kelly M. Schermerhorn, Laurie Mazzola, Joanna Bybee, Danielle Rivizzigno, Elizabeth Cantin, Barton E. Slatko and Andrew F. Gardner.

in Nucleic Acids Research

January 2016; p ublished online September 2015 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 6454 words.

Detailed biochemical characterization of nucleic acid enzymes is fundamental to understanding nucleic acid metabolism, genome replication and repair. We report the development of a rapid,...

Go to Oxford Journals »  full text: open access

The catalytic efficiency of yeast ribonuclease III depends on substrate specific product release rate

Marc-Andre Comeau, Daniel A. Lafontaine and Sherif Abou Elela.

in Nucleic Acids Research

September 2016; p ublished online June 2016 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 7172 words.

Members of the ribonuclease III (RNase III) family regulate gene expression by triggering the degradation of double stranded RNA (dsRNA). Hundreds of RNase III cleavage targets have been...

Go to Oxford Journals »  full text: open access

Comprehensive mutational scanning of a kinase <i>in vivo</i> reveals substrate-dependent fitness landscapes

Alexandre Melnikov, Peter Rogov, Li Wang, Andreas Gnirke and Tarjei S. Mikkelsen.

in Nucleic Acids Research

August 2014; p ublished online June 2014 .

Journal Article. Subjects: Research Methods in Life Sciences; Enzymology; Molecular Biology and Genetics. 4829 words.

Deep mutational scanning has emerged as a promising tool for mapping sequence–activity relationships in proteins, ribonucleic acid and deoxyribonucleic acid. In this approach, diverse...

Go to Oxford Journals »  full text: open access

<i>De novo</i> assembly of genuine replication forks on an immobilized circular plasmid in <i>Xenopus</i> egg extracts

Akiko Zembutsu and Shou Waga.

in Nucleic Acids Research

August 2006; p ublished online August 2006 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 5952 words.

We describe an improved model of DNA replication in Xenopus egg extracts, in which a circular plasmid immobilized on paramagnetic beads is used as a template. DNA synthesis occurred on...

Go to Oxford Journals »  full text: open access

Design and synthesis of fluorescent substrates for human tyrosyl-DNA phosphodiesterase I

Marc C. Rideout, Amy C. Raymond and Alex B. Burgin.

in Nucleic Acids Research

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 4942 words.

Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is a DNA repair enzyme that acts upon protein–DNA covalent complexes. Tdp1 hydrolyzes 3′-phosphotyrosyl bonds to generate 3′-phosphate DNA and free...

Go to Oxford Journals »  home page

A fluorescence-based helicase assay: application to the screening of G-quadruplex ligands

Oscar Mendoza, Nassima Meriem Gueddouda, Jean-Baptiste Boulé, Anne Bourdoncle and Jean-Louis Mergny.

in Nucleic Acids Research

June 2015; p ublished online March 2015 .

Journal Article. Subjects: Research Methods in Life Sciences; Enzymology; Molecular Biology and Genetics; Proteins. 6241 words.

Helicases, enzymes that unwind DNA or RNA structure, are present in the cell nucleus and in the mitochondrion. Although the majority of the helicases unwind DNA or RNA duplexes, some of...

Go to Oxford Journals »  full text: open access

FRET-based assay to screen inhibitors of HIV-1 reverse transcriptase and nucleocapsid protein

Kamal K. Sharma, Frédéric Przybilla, Tobias Restle, Julien Godet and Yves Mély.

in Nucleic Acids Research

May 2016; p ublished online January 2016 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 6566 words.

During HIV-1 reverse transcription, the single-stranded RNA genome is converted into proviral double stranded DNA by Reverse Transcriptase (RT) within a reverse transcription complex...

Go to Oxford Journals »  full text: open access

High-temperature single-molecule kinetic analysis of thermophilic archaeal MCM helicases

Kelly M. Schermerhorn, Nathan Tanner, Zvi Kelman and Andrew F. Gardner.

in Nucleic Acids Research

October 2016; p ublished online July 2016 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics; Proteins. 5625 words.

The minichromosome maintenance (MCM) complex is the replicative helicase responsible for unwinding DNA during archaeal and eukaryal genome replication. To mimic long helicase events in the...

Go to Oxford Journals »  full text: open access

High-throughput assay and engineering of self-cleaving ribozymes by sequencing

Shungo Kobori, Yoko Nomura, Anh Miu and Yohei Yokobayashi.

in Nucleic Acids Research

July 2015; p ublished online March 2015 .

Journal Article. Subjects: Enzymology; Research Methods in Life Sciences; Molecular Biology and Genetics. 5349 words.

Self-cleaving ribozymes are found in all domains of life and are believed to play important roles in biology. Additionally, self-cleaving ribozymes have been the subject of extensive...

Go to Oxford Journals »  full text: open access

A high-throughput assay for the comprehensive profiling of DNA ligase fidelity

Gregory J. S. Lohman, Robert J. Bauer, Nicole M. Nichols, Laurie Mazzola, Joanna Bybee, Danielle Rivizzigno, Elizabeth Cantin and Thomas C. Evans.

in Nucleic Acids Research

January 2016; p ublished online September 2015 .

Journal Article. Subjects: Enzymology; Molecular Biology and Genetics. 8044 words.

DNA ligases have broad application in molecular biology, from traditional cloning methods to modern synthetic biology and molecular diagnostics protocols. Ligation-based detection of...

Go to Oxford Journals »  full text: open access