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Cell-free TXTL synthesis of infectious bacteriophage T4 in a single test tube reaction

Mark Rustad, Allen Eastlund, Paul Jardine and Vincent Noireaux.

in Synthetic Biology

P ublished online January 2018 .

Journal Article. Subjects: Biological Engineering; Biochemistry; Bioinformatics and Computational Biology; Systems Biology; Structural Biology. 4516 words.

Abstract

The bottom-up construction of biological entities from genetic information provides a broad range of opportunities to better understand fundamental...

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Engineering DNA nanotubes for resilience in an <i>E. coli</i> TXTL system

Melissa A Klocke, Jonathan Garamella, Hari K K Subramanian, Vincent Noireaux and Elisa Franco.

in Synthetic Biology

P ublished online February 2018 .

Journal Article. Subjects: Biological Engineering; Biochemistry; Bioinformatics and Computational Biology; Systems Biology; Structural Biology. 3725 words.

Abstract

Deoxyribonucleic acid (DNA) nanotechnology is a growing field with potential intracellular applications. In this work, we use an Escherichia coli cell...

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A set of experimentally validated, mutually orthogonal primers for combinatorially specifying genetic components

Subu K Subramanian, William P Russ and Rama Ranganathan.

in Synthetic Biology

P ublished online January 2018 .

Journal Article. Subjects: Biological Engineering; Biochemistry; Bioinformatics and Computational Biology; Systems Biology; Structural Biology. 2187 words.

Abstract

The design and synthesis of novel genes and deoxyribonucleic acid (DNA) sequences is a central technique in synthetic biology. Current methods of high...

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