Journal Article

Formation of Benzoylecgonine Isopropyl Ester Following Solid-Phase Extraction

Matthew Juhascik, Jessica Gleba and Amanda Jenkins

in Journal of Analytical Toxicology

Volume 36, issue 2, pages 141-143
Published in print March 2012 | ISSN: 0146-4760
Published online March 2012 | e-ISSN: 1945-2403 | DOI:
Formation of Benzoylecgonine Isopropyl Ester Following Solid-Phase Extraction

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  • Medical Toxicology
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In forensic toxicology general alkaline drug screens typically utilize liquid liquid [LLE] or solid phase extraction [SPE] sample preparation techniques. It is expected that different drugs will be detected when a laboratory changes techniques. In this study, when the authors switched from LLE to SPE they were able to detect benzoylecgonine [BE]. Benzoylecgonine isopropyl ester [BEIE] was also detected. Further investigation demonstrated that the BEIE was formed during sample elution with methylene chloride/isopropanol/ammonium hydroxide. BEIE was not detected if methanol/ammonium hydroxide was used as the elution solvent. Analysts should be aware that BEIE is formed in the presence of BE if elution solvents comprise isopropanol and a strong base. Therefore, use of BEIE as an internal standard in such assays will result in inaccurate quantitation of BE.

Journal Article.  1025 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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