Journal Article

<i>OARE-1</i>, a <i>Ty1-copia</i> Retrotransposon in Oat Activated by Abiotic and Biotic Stresses

Yosuke Kimura, Yukio Tosa, Saori Shimada, Ryohei Sogo, Motoaki Kusaba, Tetsuo Sunaga, Shigeyuki Betsuyaku, Yukiko Eto, Hitoshi Nakayashiki and Shigeyuki Mayama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 12, pages 1345-1354
Published in print December 2001 | ISSN: 0032-0781
Published online December 2001 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pce171
OARE-1, a Ty1-copia Retrotransposon in Oat Activated by Abiotic and Biotic Stresses

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Transcriptionally active Ty1-copia LTR-retrotransposons were found in oat using RT-PCR for amplifying the reverse transcriptase domain. Sequence analysis of the RT-PCR clones suggested that oat LTR-retrotransposons consist of at least seven groups, which were tentatively designated as Oatrt1 to Oatrt7. A full length copy of Oatrt1 was isolated from an oat genomic library, and was designated OARE-1. OARE-1 was 8,665 bp long and a member of the BARE-1 subgroup. The oat genome carried it in multiple copies (at least 10,000 copies / a hexaploid genome). The expression of OARE-1 was intensively induced by wounding, UV light, jasmonic acid and salicylic acid, and its pattern was very similar to that of the PAL (phenylalanin ammonia lyase) gene. Furthermore, OARE-1 was highly activated by infection with an incompatible race of the crown rust fungus, Puccinia coronata. These results suggest that OARE-1 is highly sensitive to various abiotic and biotic stimuli leading to plant defense responses.

Keywords: Key words: Avena sativa— Oat — Retrotransposon — Transposable element.

Journal Article.  6459 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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