Journal Article

RiceFOX: A Database of Arabidopsis Mutant Lines Overexpressing Rice Full-Length cDNA that Contains a Wide Range of Trait Information to Facilitate Analysis of Gene Function

Tetsuya Sakurai, Youichi Kondou, Kenji Akiyama, Atsushi Kurotani, Mieko Higuchi, Takanari Ichikawa, Hirofumi Kuroda, Miyako Kusano, Masaki Mori, Tsutomu Saitou, Hitoshi Sakakibara, Shoji Sugano, Makoto Suzuki, Hideki Takahashi, Shinya Takahashi, Hiroshi Takatsuji, Naoki Yokotani, Takeshi Yoshizumi, Kazuki Saito, Kazuo Shinozaki, Kenji Oda, Hirohiko Hirochika and Minami Matsui

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 52, issue 2, pages 265-273
Published in print February 2011 | ISSN: 0032-0781
Published online December 2010 | e-ISSN: 1471-9053 | DOI: https://dx.doi.org/10.1093/pcp/pcq190

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Identification of gene function is important not only for basic research but also for applied science, especially with regard to improvements in crop production. For rapid and efficient elucidation of useful traits, we developed a system named FOX hunting (Full-length cDNA Over-eXpressor gene hunting) using full-length cDNAs (fl-cDNAs). A heterologous expression approach provides a solution for the high-throughput characterization of gene functions in agricultural plant species. Since fl-cDNAs contain all the information of functional mRNAs and proteins, we introduced rice fl-cDNAs into Arabidopsis plants for systematic gain-of-function mutation. We generated >30,000 independent Arabidopsis transgenic lines expressing rice fl-cDNAs (rice FOX Arabidopsis mutant lines). These rice FOX Arabidopsis lines were screened systematically for various criteria such as morphology, photosynthesis, UV resistance, element composition, plant hormone profile, metabolite profile/fingerprinting, bacterial resistance, and heat and salt tolerance. The information obtained from these screenings was compiled into a database named ‘RiceFOX’. This database contains around 18,000 records of rice FOX Arabidopsis lines and allows users to search against all the observed results, ranging from morphological to invisible traits. The number of searchable items is approximately 100; moreover, the rice FOX Arabidopsis lines can be searched by rice and Arabidopsis gene/protein identifiers, sequence similarity to the introduced rice fl-cDNA and traits. The RiceFOX database is available at http://ricefox.psc.riken.jp/.

Keywords: Arabidopsis thaliana; Full-length cDNA; Gene function; Oryza sativa; Trait; Transgenic plant

Journal Article.  4325 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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