Journal Article

An Initial Characterization of N-Terminal-Proatrial Natriuretic Peptide in Serum of Sprague Dawley Rats

Heidi M. Colton, Alan H. Stokes, Lawrence W. Yoon, Michael P. Quaile, Paul J. Novak, James Greg Falls, Carie L. Kimbrough, Neal F. Cariello, Holly L. Jordan and Brian R. Berridge

in Toxicological Sciences

Volume 120, issue 2, pages 262-268
Published in print April 2011 | ISSN: 1096-6080
Published online January 2011 | e-ISSN: 1096-0929 | DOI:
An Initial Characterization of N-Terminal-Proatrial Natriuretic Peptide in Serum of Sprague Dawley Rats

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  • Medical Toxicology
  • Toxicology (Non-medical)


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In the clinical setting, natriuretic peptides (NPs) have proven to be reliable noninvasive markers for diagnostic, prognostic, and therapeutic monitoring of heart failure. Given their proven utility in humans, NPs are potential candidates for translational biomarkers during drug development to detect drug-induced hemodynamic stress resulting in cardiac hypertrophy in preclinical species. We evaluated the intra- and interassay precision and the stability of serum N-terminal-proatrial natriuretic peptide (NT-proANP) using a commercially available enzyme-linked immunoassay (EIA). We then measured NT-proANP concentrations in 532 serum samples from 337 male Crl:CD(SD) rats with or without pressure-induced cardiac hypertrophy. Additionally, we established a reference range using samples from control animals across multiple studies. The data demonstrate that the NT-proANP EIA is a robust and reproducible assay for the measurement of NT-proANP. The noninvasive translational utility, minimal sample volume requirement, and the lack of existing hypertrophic biomarkers in the male rat make NT-proANP an excellent candidate for further interrogation as a biomarker of cardiac hypertrophy in preclinical toxicology investigations.

Keywords: NT-proANP; natriuretic peptide; rat; preclinical toxicology

Journal Article.  4415 words.  Illustrated.

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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