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phosphoramidite

Overview page. Subjects: Chemistry — Medicine and Health.

Casual term used by molecular biologists for nucleoside phosphoramidites in which a phosphite with an amino group is substituted for one of the hydroxyls, instead of the normal phosphate....

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phosphoramidite

Overview page. Subjects: Chemistry — Medicine and Health.

Casual term used by molecular biologists for nucleoside phosphoramidites in which a phosphite with an amino group is substituted for one of the hydroxyls, instead of the normal phosphate....

See overview in Oxford Index

phosphoramidite

John Lackie.

in A Dictionary of Biomedicine

January 2010; p ublished online January 2010 .

Reference Entry. Subjects: Clinical Medicine. 41 words.

Casual term used by molecular biologists for nucleoside phosphoramidites in which a phosphite with an amino group is substituted for

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phosphoramidite

Edited by Richard Cammack, Teresa Atwood, Peter Campbell, Howard Parish, Anthony Smith, Frank Vella and John Stirling.

in Oxford Dictionary of Biochemistry and Molecular Biology

January 2006; p ublished online January 2008 .

Reference Entry. Subjects: Biochemistry. 44 words.

any compound having the general structure R3R4NP(R2)−O−R1. Phosphoramidites are useful in the

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Codon based mutagenesis using trimer phosphoramidites

John Randolph, Andrey Yagodkin, Marc Lamaitre, Alex Azhayev and Hugh Mackie.

in Nucleic Acids Symposium Series

September 2008; p ublished online September 2008 .

Journal Article. Subjects: Chemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 0 words.

A set of Trimer phosphoramidites was synthesized covering all 20 amino acid codons. These trimer phosphoramidites can be added during synthesis using standard DNA chemistry. A Reaction...

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Tandem oligonucleotide synthesis using linker phosphoramidites

Richard T. Pon and Shuyuan Yu.

in Nucleic Acids Research

January 2005; p ublished online January 2005 .

Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 6116 words.

Multiple oligonucleotides of the same or different sequence, linked end-to-end in tandem can be synthesized in a single automated synthesis. A linker phosphoramidite [R. T. Pon and S. Yu...

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Tandem oligonucleotide synthesis using linker (First Base™) phosphoramidites

Richard T. Pon and Shuyuan Yu.

in Nucleic Acids Symposium Series

November 2002; p ublished online November 2002 .

Journal Article. Subjects: Chemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 0 words.

First Base™ linker phosphoramidites add the first nucleoside to underivatized solid-phase supports using a regular phosphoramidite coupling cycle. A cleavable 3′-O-ester linkage allows NH4O...

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Codon-based mutagenesis using dimer-phosphoramidites

Philippe Neuner, Riccardo Cortese and Paolo Monaci.

in Nucleic Acids Research

March 1998; p ublished online March 1998 .

Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 2996 words.

A new approach for the synthesis of randomized DNA sequences containing the 20 codons corresponding to all natural amino acids is described. The strategy is based on the use of dinucleotide...

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Photocleavable aminotag phosphoramidites for 5′-termini DNA/RNA labeling

Jerzy Olejnik, Edyta Krzymanska-Olejnik and Kenneth J. Rothschild.

in Nucleic Acids Research

August 1998; p ublished online August 1998 .

Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 2782 words.

We report the design and evaluation of two non-nucleosidic photocleavable aminotag phosphor-amidites. These reagents introduce a photocleavable amino group on the 5′-terminal phosphate of...

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Internucleotide-linkage formation via the phosphoramidite method using a carboxylic acid as a promoter

Masaki Tsukamoto, Erkki J. Nurminen, Toshihiro Iwase, Masanori Kataoka and Yoshihiro Hayakawa.

in Nucleic Acids Symposium Series

November 2004; p ublished online November 2004 .

Journal Article. Subjects: Chemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 0 words.

This paper describes utility of a carboxylic acid, such as 2,4-dinitrobenzoic acid, as a promoter for internucleotide-linkage formation via the phosphoramidite method.

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Linker phosphoramidite reagents for the attachment of the first nucleoside to underivatized solid‐phase supports

Richard T. Pon and Shuyuan Yu.

in Nucleic Acids Research

January 2004; p ublished online January 2004 .

Journal Article. Subjects: Biological Sciences. 6641 words.

New linker phosphoramidite reagents containing a cleavable 3′‐ester linkage are used for attaching the first nucleoside to the surface of a solid‐ phase support. Inexpensive, underivatized...

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