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spheroplast

Overview page. Subjects: Chemistry.

A protoplast (q.v.) to which some cell wall remnants are attached. For example, a rod-shaped bacterium treated with lysozyme becomes spherical because the enzyme removes peptidoglycan...

See overview in Oxford Index

spheroplast

Overview page. Subjects: Chemistry.

A protoplast (q.v.) to which some cell wall remnants are attached. For example, a rod-shaped bacterium treated with lysozyme becomes spherical because the enzyme removes peptidoglycan...

See overview in Oxford Index

spheroplast

Robert C. King, William D. Stansfield and Pamela K. Mulligan.

in A Dictionary of Genetics

January 2007; p ublished online January 2007 .

Reference Entry. Subjects: Genetics and Genomics. 35 words.

a protoplast (q.v.) to which some cell wall remnants are attached. For example, a rod-shaped bacterium treated with

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spheroplast

Robert C. King, Pamela K. Mulligan and William D. Stansfield.

in A Dictionary of Genetics

January 2013; p ublished online January 2014 .

Reference Entry. Subjects: Genetics and Genomics. 35 words.

a protoplast (q.v.) to which some cell wall remnants are attached. For example, a rod-shaped bacterium treated with

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spheroplast

Edited by Richard Cammack, Teresa Atwood, Peter Campbell, Howard Parish, Anthony Smith, Frank Vella and John Stirling.

in Oxford Dictionary of Biochemistry and Molecular Biology

January 2006; p ublished online January 2008 .

Reference Entry. Subjects: Biochemistry. 60 words.

the globular form of a bacterial cell in which the cell‐wall structure has been modified (e.g. by growth in the

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Variations in mRNA transcript levels of cell wall-associated genes of <i>Saccharomyces cerevisiae</i> following spheroplasting

Richard Braley and W. LaJean Chaffin.

in FEMS Microbiology Letters

December 1999; p ublished online January 2006 .

Journal Article. 3339 words.

Abstract

mRNA transcript levels of 38 genes from Saccharomyces cerevisiae were investigated during attempted spheroplast regeneration. Many of the genes...

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sphero-

Elizabeth Martin.

in The New Oxford Dictionary for Scientific Writers and Editors

January 2009; p ublished online January 2009 .

Reference Entry. Subjects: Language Reference. 20 words.

Prefix denoting a sphere, spherical or near-spherical, or curvature (e.g. spherometer, spheroplast, spherosome). See also sphaero-.

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Detection of a Compact Folding Intermediate of Dimethyl Sulfoxide Reductase Secreted from a Molybdenum Cofactor-Deficient Mutant of<i>Rhodobacter sphaeroides</i>f. sp. denitrificans

Masahiro Matsuzaki and Toshio Satoh.

in Plant and Cell Physiology

January 1997; p ublished online January 1997 .

Journal Article. Subjects: Biochemistry; Molecular and Cell Biology; Plant Sciences and Forestry. 0 words.

All of the nine cysteine residues in dimethyl sulfoxide reductase (OMSOR) exist in reduced thiol form. The unfolded form, which was previously detected in DMSOR proteins secreted by...

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Effects of <i>ortho</i>-phthalaldehyde, glutaraldehyde and chlorhexidine diacetate on <i>Mycobacterium chelonae</i> and <i>Mycobacterium abscessus</i> strains with modified permeability

S. Fraud, A. C. Hann, J.-Y. Maillard and A. D. Russell.

in Journal of Antimicrobial Chemotherapy

March 2003; p ublished online March 2003 .

Journal Article. Subjects: Medical Oncology; Critical Care. 5676 words.

The mechanisms of the mycobactericidal action of ortho-phthalaldehyde (OPA), glutaraldehyde (GTA) and chlorhexidine diacetate (CHA) were investigated using mycobacterial spheroplasts of two...

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Isolation of nuclei for chromatin analysis in fission yeast

Jacqueline A. Mason and Jane Mellor.

in Nucleic Acids Research

November 1997; p ublished online November 1997 .

Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 1305 words.

The methods available for analysis of the chromatin of Schizosaccharomyces pombe are time consuming (>8 h) and/or result in some degradation of the chromatin. Here we report an optimised...

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Single cell studies of enzymatic hydrolysis of a tetramethylrhodamine labeled triglucoside in yeast

X. Chris Le, Woei Tan, Christine H. Scaman, Adam Szpacenko, Edgar Arriaga, Yanni Zhang, Norman J. Dovichi, Ole Hindsgaul and Monica M. Palcic.

in Glycobiology

March 1999; p ublished online March 1999 .

Journal Article. Subjects: Carbohydrates. 4357 words.

Several hundred molecules of enzyme reaction products were detected in a single spheroplast from yeast cells incubated with a tetramethylrhodamine (TMR) labeled triglucoside, α-d-Glc(1→2)α-dnull...

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Intracellular location of catalase-peroxidase hydroperoxidase I of <i>Escherichia coli</i>

Alex Hillar, Larry Van Caeseele and Peter C. Loewen.

in FEMS Microbiology Letters

January 1999; p ublished online January 2006 .

Journal Article. 2384 words.

Abstract

The catalase-peroxidase hydroperoxidase I of Escherichia coli has been confirmed to be located in the cytoplasm using two independent methods....

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Inorganic polyphosphate in mitochondria of <i>Saccharomyces cerevisiae</i> at phosphate limitation and phosphate excess

Nikolay A Pestov, Tatyana V Kulakovskaya and Igor S Kulaev.

in FEMS Yeast Research

March 2004; p ublished online January 2006 .

Journal Article. 2816 words.

Abstract

Isolated mitochondria of Saccharomyces cerevisiae cells grown on glucose possess acid-soluble inorganic polyphosphate (polyP). Its level strongly...

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Aspects of the antimicrobial mechanisms of action of a polyquaternium and an amidoamine

Caroline E. Codling, Jean-Yves Maillard and A. Denver Russell.

in Journal of Antimicrobial Chemotherapy

May 2003; p ublished online May 2003 .

Journal Article. Subjects: Medical Oncology; Critical Care. 3099 words.

Objectives: Polyquad (Alcon) (polyquaternium-1, PQ-1) and Aldox (Alcon) (myristamidopropyl dimethylamine, MAPD) are two biocides that are used commercially in a contact lens...

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Quinolone accumulation by <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>

Laura J. V. Piddock, Y.-F. Jin, V. Ricci and Anne E. Asuquo.

in Journal of Antimicrobial Chemotherapy

January 1999; p ublished online January 1999 .

Journal Article. Subjects: Medical Oncology; Critical Care. 6056 words.

The accumulation of nalidixic acid and 14 fluoroquinolones over a range of external drug concentrations (10–100 mg/L; c. 25–231 µM) into intact cells of Escherichia coli KL-16, Staphylococ...

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Resistance of Gram-positive bacteria to nisin is not determined by Lipid II levels

Naomi E. Kramer, Eddy J. Smid, Jan Kok, Ben de Kruijff, Oscar P. Kuipers and Eefjan Breukink.

in FEMS Microbiology Letters

October 2004; p ublished online January 2006 .

Journal Article. 2867 words.

Abstract

Lipid II is essential for nisin-mediated pore formation at nano-molar concentrations. We tested whether nisin resistance could result from different...

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Studies of the subtilin leader peptide as a translocation signal in <i>Escherichia coli</i> K12

Leena K. Paul, Gonzalo Izaguirre and J. Norman Hansen.

in FEMS Microbiology Letters

July 1999; p ublished online January 2006 .

Journal Article. 2682 words.

Abstract

Subtilin is an antimicrobial peptide of the lantibiotic family that is produced by Gram-positive Bacillus subtilis, and its biosynthesis involves...

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In <i>Saccharomyces cerevisiae</i> gene targeting fidelity depends on a transformation method and proportion of the overall length of the transforming and targeted DNA

Anamarija Štafa, Marina Svetec Miklenić, Antonio Zandona, Bojan Žunar, Neža Čadež, Hrvoje Petković and Ivan Krešimir Svetec.

in FEMS Yeast Research

P ublished online June 2017 .

Journal Article. Subjects: Biotechnology; Genetics and Genomics; Microbiology; Molecular and Cell Biology; Mycology and Fungi. 6644 words.

Abstract

Gene replacement is one of the most essential approaches in construction of the genetically modified yeast strains. However, the fidelity of gene...

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Optimum conditions for selective isolation of genes from complex genomes by transformation‐associated recombination cloning

Sun‐Hee Leem, Vladimir N. Noskov, Jung‐Eun Park, Seung Il Kim, Vladimir Larionov and Natalay Kouprina.

in Nucleic Acids Research

March 2003; p ublished online March 2003 .

Journal Article. Subjects: Chemistry; Biochemistry; Bioinformatics and Computational Biology; Genetics and Genomics; Molecular and Cell Biology. 4738 words.

Transformation‐associated recombination (TAR) cloning in yeast is used to isolate a desired chromosomal region or gene from a complex genome without construction of a genomic library. The...

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Extensive MHC class I-restricted CD8 T lymphocyte responses against various yeast genera in humans

Tanja Heintel, Frank Breinig, Manfred J. Schmitt and Andreas Meyerhans.

in FEMS Immunology & Medical Microbiology

December 2003; p ublished online January 2006 .

Journal Article. Subjects: Medical Microbiology and Virology; Biotechnology; Genetics and Genomics; Microbiology; Molecular and Cell Biology. 5066 words.

Abstract

The human cellular immune response against 14 distantly related yeast species was analyzed by intracellular cytokine staining of lymphocytes after...

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Ca<sup>2+</sup>-transporting ATPase(s) of the reticulum type in intracellular membranes of <i>Saccharomyces cerevisiae</i>: biochemical identification

Lev A Okorokov, Andrei Ju Kuranov, Evgenia V Kuranova and Robson dos Santos Silva.

in FEMS Microbiology Letters

January 1997; p ublished online January 2006 .

Journal Article. 3862 words.

Abstract

Several lines of evidence are presented to show that the Ca2+-ATPase activity of total yeast membranes is due to the reticulum (R) type of Ca2+-ATPa...

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