Journal Article

Phylogenetic Analysis of Dipterocarps Using Random Amplified Polymorphic DNA Markers

PRIYADARSHINI RATH, G. RAJASEGER, CHONG JIN GOH and PRAKASH P. KUMAR

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 82, issue 1, pages 61-65
Published in print July 1998 | ISSN: 0305-7364
Published online July 1998 | e-ISSN: 1095-8290 | DOI: https://dx.doi.org/10.1006/anbo.1998.0652
Phylogenetic Analysis of Dipterocarps Using Random Amplified Polymorphic DNA Markers

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The phylogenetic relationships among 12 species belonging to three different genera (Shorea, HopeaandAnisoptera) of Dipterocarpaceae were studied using random amplified polymorphic DNA (RAPD) markers. A modified CTAB DNA extraction protocol was used to obtain tannin- and polysaccharide-free genomic DNA from mature leaves. Cluster analysis of data from six random primers placed the 12 species in three groups corresponding to their respective genera. Four distinct nodes ofShoreaspp. and two ofHopeaspp. could be identified.Anisoptera megistocarpaserved as an outgroup, and was unique when compared to the other genera examined. RAPD profiles of five individuals ofH. odoratawith six random primers were identical, suggesting that there is little intraspecific variation in this species. The RAPD technique can thus be successfully applied for the study of phylogenetic relationships of this important group of tropical timber trees.Copyright 1998 Annals of Botany Company

Keywords: Dipterocarpaceae,Anisopteraspp.,Hopeaspp.,Shoreaspp., RAPD markers, tropical timber trees.

Journal Article.  7 words. 

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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