Journal Article

Detection of free polyamine in coastal seawater using ion exchange chromatography

Naoyoshi Nishibori, Akinori Nishii and Haruyoshi Takayama

in ICES Journal of Marine Science

Published on behalf of ICES/CIEM

Volume 58, issue 6, pages 1201-1207
Published in print January 2001 | ISSN: 1054-3139
Published online January 2001 | e-ISSN: 1095-9289 | DOI: http://dx.doi.org/10.1006/jmsc.2001.1115
Detection of free polyamine in coastal seawater using ion exchange chromatography

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Polyamines are widely distributed in living organisms and are known to be essential elements for normal growth and development. Although they may affect phytoplankton growth their existence and concentration in seawater is unclear. In this paper the polyamine concentrations in coastal seawater are measured by high performance liquid chromatography (HPLC) using cation exchange resin and OPA reagent. This method seems sufficient for their detection in seawater. Putrescine and spermidine were found to be the major polyamines in the coastal seawater, though their respective concentrations varied widely. In addition to them cadaverine, norspermidine, norspermine, and spermine were also detected.

Keywords: polyamine; seawater; HPLC; cation exchange resin

Journal Article.  0 words. 

Subjects: Environmental Science ; Marine and Estuarine Biology

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