Journal Article

Carbon repression of cellobiose dehydrogenase production in the white rot fungus <i>Trametes versicolor</i> is mediated at the level of gene transcription

P.C. Stapleton and A.D.W. Dobson

in FEMS Microbiology Letters

Volume 221, issue 2, pages 167-172
Published in print April 2003 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1016/S0378-1097(03)00212-X

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Abstract

Cellobiose dehydrogenase (CDH) production in Trametes versicolor is induced in the presence of cellulose, but decreases when additional carbon sources such as glucose and maltose are added to the fungal cultures. Using T. versicolor-specific cdh primers in a reverse transcription-polymerase chain reaction-based approach, it appears that this repression in CDH production is being mediated at the level of gene transcription. When a 1.6-kb upstream region of the T. versicolor cdh gene was cloned and sequenced, a number of putative CreA-like binding sites were observed. We propose that these sites may be involved in mediating this repressive effect, based on their similarity to the consensus [5′-SYGGRGG-3′] site for binding of the CreA and Cre1 repressor proteins.

Keywords: Cellobiose dehydrogenase; Trametes versicolor; Repression

Journal Article.  3305 words.  Illustrated.

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