Journal Article

Endothelial cell seeding improves patency of synthetic vascular grafts: manual versus automatized method

in European Journal of Cardio-Thoracic Surgery

Published on behalf of European Association for Cardio-Thoracic Surgery

Volume 10, issue 5, pages 372-379
Published in print May 1996 | ISSN: 1010-7940
Published online May 1996 | e-ISSN: 1873-734X | DOI:
Endothelial cell seeding improves patency of synthetic vascular grafts: manual versus automatized method

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  • Cardiovascular Medicine
  • Cardiothoracic Surgery


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Lack of an endothelial surface is the most important variable causingthe relatively poor patency of synthetic bypass grafts. This study wasdesigned to investigate the effect of endothelial cell seeding on small-diameter Dacron grafts seeded with microvascular endothelial cells fromomentum, and to evaluate two methods (manual vs automatized) for one- stageseeding in a canine carotid artery model. In 30 mongrel dogs microvascularendothelial cells were harvested from omentum, either by a manual or anautomatized method, and seeded onto 6-mm internal diameter Dacronprostheses prior to the graft interposition into the common carotidarteries. Non-seeded Dacron grafts were used as control grafts. All dogsreceived dipyridamole (75 mg/day) and acetylsalicylic acid (325 mg/day) for4 weeks. The prostheses were explanted between 2 and 26 weeks afterinsertion. The results were assessed by patency, angiography, light andscanning electron microscopy, transmission electron microscopy, andmorphometry. Endothelial cell seeding improved the patency ratesignificantly, regardless of the seeding methods used. The overallactuarial patency rates at 5, 12, and 26 weeks were 98%, 94% and 94%,respectively, for the seeded Dacron grafts, and 92%, 62% and 54%,respectively, for the non-seeded grafts. The automatized method yieldedmore endothelial cells per gram of omental tissue than the manual method (P= 0.0002), but there was no difference (P = 0.34) between the seedingdensities per square centimeter of the graft surface. The harvesting andseeding by the automatized method took 55 min for the whole procedure, 20min less than the manual method. We concluded that one-stage endothelialcell seeding with omental microvascular endothelial cells improved thepatency of small-diameter Dacron grafts in a canine model. The automatizedmethod obtained excellent results comparable to the manual procedure, andalso reduced the time necessary for the cell seeding.

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Subjects: Cardiovascular Medicine ; Cardiothoracic Surgery

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