Journal Article

Gamma delta T cell responses associated with the development of tuberculosis in health care workers

Diane J. Ordway, Luisa Pinto, Leonor Costa, Marta Martins, Clara Leandro, Miguel Viveiros, Leonard Amaral, Maria J. Arroz, Fernando A. Ventura and Hazel M. Dockrell

in FEMS Immunology & Medical Microbiology

Published on behalf of Federation of Microbiological Societies

Volume 43, issue 3, pages 339-350
Published in print March 2005 | ISSN: 0928-8244
Published online January 2006 | e-ISSN: 1574-695X | DOI: http://dx.doi.org/10.1016/j.femsim.2004.09.005
Gamma delta T cell responses associated with the development of tuberculosis in health care workers

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Abstract

This study evaluated T cell immune responses to purified protein derivative (PPD) and Mycobacterium tuberculosis (Mtb) in health care workers who remained free of active tuberculosis (HCWs w/o TB), health care workers who went on to develop active TB (HCWs w/TB), non-health care workers who were TB free (Non-HCWs) and tuberculosis patients presenting with minimal (Min TB) or advanced (Adv TB) disease. Peripheral blood mononuclear cells (PBMC) were stimulated with Mtb and PPD and the expression of T cell activation markers CD25+ and HLA-DR+, intracellular IL-4 and IFN-γ production and cytotoxic responses were evaluated. PBMC from HCWs who developed TB showed decreased percentages of cells expressing CD8+CD25+ in comparison to HCWs who remained healthy. HCWs who developed TB showed increased γδ TCR+ cell cytotoxicity and decreased CD3+γδ TCR+ cell cytotoxicity in comparison to HCWs who remained healthy. PBMC from TB patients with advanced disease showed decreased percentages of CD25+CD4+ and CD25+CD8+ T cells that were associated with increased IL-4 production in CD8+ and γδ TCR+ phenotypes, in comparison with TB patients presenting minimal disease. TB patients with advanced disease showed increased γδ TCR+ cytotoxicity and reduced CD3+γδ TCR+ cell cytotoxicity. Our results suggest that HCWs who developed TB show an early compensatory mechanism involving an increase in lytic responses of γδ TCR+ cells which did not prevent TB.

Keywords: M. tuberculosis; γδ T cells; Health care workers

Journal Article.  6974 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Biotechnology ; Genetics and Genomics ; Microbiology ; Molecular and Cell Biology

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