Journal Article

A mitochondrial DNA PCR–RFLP marker for population studies of the black scabbardfish (<i>Aphanopus carbo</i>)

Ricardo Quinta, Laurentina Gomes and Ana Teia dos Santos

in ICES Journal of Marine Science

Published on behalf of ICES/CIEM

Volume 61, issue 5, pages 864-867
Published in print January 2004 | ISSN: 1054-3139
Published online January 2004 | e-ISSN: 1095-9289 | DOI: http://dx.doi.org/10.1016/j.icesjms.2004.03.003
A mitochondrial DNA PCR–RFLP marker for population studies of the black scabbardfish (Aphanopus carbo)

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Black scabbardfish (Aphanopus carbo), a commercially valuable marine fish off Portugal and the Madeira Archipelago, was surveyed for mitochondrial DNA variation of part of the cytochrome b gene. In all, 51 fish from three Northeast Atlantic localities were examined using ten restriction enzymes. Overall nucleon diversity was 0.180. Genetic differentiation (θ=0.25) was significant; the Madeira Archipelago sample was distinguishable from samples from the other two localities. The approach should be useful for a more extensive study of black scabbardfish populations.

Keywords: Aphanopus carbo; PCR–RFLP; stock identification

Journal Article.  1856 words.  Illustrated.

Subjects: Environmental Science ; Marine and Estuarine Biology

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