Journal Article

Novel DNA markers for rapid, accurate, and cost-effective discrimination of the continental origin of Atlantic salmon (<i>Salmo salar</i> L.)

John Gilbey, David Knox, Martha O'Sullivan and Eric Verspoor

in ICES Journal of Marine Science

Published on behalf of ICES/CIEM

Volume 62, issue 8, pages 1606-1616
Published in print January 2005 | ISSN: 1054-3139
Published online January 2005 | e-ISSN: 1095-9289 | DOI: http://dx.doi.org/10.1016/j.icesjms.2005.07.001
Novel DNA markers for rapid, accurate, and cost-effective discrimination of the continental origin of Atlantic salmon (Salmo salar L.)

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Salmon from geographically representative rivers in North America and Europe were typed for variation at the microsatellite locus SS1 and the mitochondrial DNA ND-1 restriction site 3971, using PCR amplification and agarose-gel electrophoresis. North American salmon showed near-fixation for SS1 alleles between 129 and 135 bp in length and the N mtDNA restriction type, while European salmon near-fixation for SS1 alleles between 183 and 219 bp and the A/D mtDNA type. Based on the observed variant frequencies, using these two markers in combination is predicted to give correct assignment of >99.5% of salmon to continent-of-origin. As both these continental markers can be screened by agarose-gel electrophoresis, their use offers a more rapid, cheaper, and simpler method for accurate assignment of Atlantic salmon to continent-of-origin than do existing methods. These markers can be applied to the identification of salmon in North Atlantic high-seas fisheries, in aquaculture stocks, and in rivers to determine the continent-of-origin of fish-farm escapes.

Keywords: continental origin; microsatellite marker; mtDNA; stock discrimination

Journal Article.  4472 words.  Illustrated.

Subjects: Environmental Science ; Marine and Estuarine Biology

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