Journal Article

Activated lymphocytes reduce adherence of <i>Aspergillus fumigatus</i>

M.D. Martins, L.J. Rodriguez, C.A. Savary, M.L. Grazziutti, D. Deshpande, D.M. Cohen, R.E. Cowart, D.G. Woodside, B.W. Mcintyre, E.J. Anaissie and J.H. Rex

in Medical Mycology

Published on behalf of International Society for Human and Animal Mycology

Volume 36, issue 5, pages 281-289
Published in print January 1998 | ISSN: 1369-3786
Published online January 1998 | e-ISSN: 1460-2709 | DOI: http://dx.doi.org/10.1080/02681219880000451
Activated lymphocytes reduce adherence of Aspergillus fumigatus

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  • Mycology and Fungi
  • Infectious Diseases
  • Medical Toxicology
  • Veterinary Medicine
  • Environmental Science

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Lymphocytes comprise up to 30% of the cells present in human bronchoalveolar lavage fluid and thus could participate in host response to infectious Aspergillus fumigatus conidia. We have examined the possibility that lymphocytes might play a role during early infection by either damaging the fungus or interfering with adherence. When incubated with A. fumigatus conidia for 20 h, highly purified 5-day-old lymphocytes activated with either IL-2 or phytohaemagglutinin, but not untreated lymphocytes, were consistently able to reduce residual fungal biomass as estimated by a metabolic assay. T lymphocytes, but not NK cells, appeared to be responsible for this activity. Lymphocytes bound both A. fumigatus conidia and hyphae, and the antifungal activity of the lymphocytes required direct lymphocyte-fungus contact. In a separate set of experiments using release of 51Cr from 51 Cr-loaded fungi as an estimate of fungal damage, lymphocyte-induced loss of fungal biomass was foundto be due to loss of fungal adherence rather than to direct fungal damage. The detached hyphae were also found to be metabolically intact and to have normal morphology by electron microscopy. Thesedata demonstrate that IL-2- and phytohaemagglutinin-activated lymphocytes exhibit a contact-dependent ability to reduce adherence of germinating conidia of A. fumigatus to a surface.

Keywords: Aspergillus; lymphocytes; host defence

Journal Article.  0 words. 

Subjects: Mycology and Fungi ; Infectious Diseases ; Medical Toxicology ; Veterinary Medicine ; Environmental Science

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