Journal Article

Molecular Evolutionary Analysis of the Complete Nucleotide Sequence of Hepatitis B Virus (HBV) in a Case of HBV Infection Acquired through a Needlestick Accident

Fuminaka Sugauchi, Masashi Mizokami, Etsuro Orito, Tomoyoshi Ohno, Katsuo Hayashi, Takanobu Kato, Yasuhito Tanaka, Hideaki Kato and Ryuzo Ueda

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 31, issue 5, pages 1195-1201
Published in print November 2000 | ISSN: 1058-4838
Published online November 2000 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/317428
Molecular Evolutionary Analysis of the Complete Nucleotide Sequence of Hepatitis B Virus (HBV) in a Case of HBV Infection Acquired through a Needlestick Accident

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To elucidate needlestick transmission of hepatitis B virus (HBV), strains isolated from 1 physician who acquired HBV infection through a needlestick accident and 3 patients with chronic hepatitis B (donor patients A, B, and C) were tested using molecular evolutionary analysis based on full-length HBV genomic sequences. Nucleotide sequences of these isolates were aligned with 55 previously reported full-length genomic sequences. Genetic distances were estimated using the 6-parameter method, and phylogenetic trees were constructed using the neighbor-joining method. Strains isolated from patient A and the recipient pair were clustered within a closer range of evolutionary distances than were strains recovered from the recipient pair and patients B and C. Furthermore, strains from patient A and the recipient were also clustered on the S gene sequences of HBV. These results demonstrated that patient A alone was the source of direct transmission to the recipient. This approach can be used to investigate the transmission route of HBV.

Journal Article.  3089 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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