Journal Article

<i>Mycobacterium tuberculosis</i> Complex DNA from an Extinct Bison Dated 17,000 Years before the Present

Bruce M. Rothschild, Larry D. Martin, Galit Lev, Helen Bercovier, Gila Kahila Bar-Gal, Charles Greenblatt, Helen Donoghue, Mark Spigelman and David Brittain

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 33, issue 3, pages 305-311
Published in print August 2001 | ISSN: 1058-4838
Published online August 2001 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/321886
Mycobacterium tuberculosis Complex DNA from an Extinct Bison Dated 17,000 Years before the Present

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In order to assess the presence of tuberculosis in Pleistocene bison and the origin of tuberculosis in North America, 2 separate DNA extractions were performed by 2 separate laboratories on samples from the metacarpal of an extinct long-horned bison that was radiocarbon dated at 17,870 ± 230 years before present and that had pathological changes suggestive of tuberculosis. Polymerase chain reaction amplification isolated fragments of tuberculosis DNA, which were sequenced, and on which spoligotyping was also performed to help determine its relationship to the various members of the Mycobacterium tuberculosis complex. Extensive precautions against contamination with modern M. tuberculosis complex DNA were employed, including analysis of paleontologic and modern specimens in 2 geographically separate laboratories.

Journal Article.  4700 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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