Journal Article

Standardizing <i>Chlamydia pneumoniae</i> Assays: Recommendations from the Centers for Disease Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada)

Scott F. Dowell, Rosanna W. Peeling, Jens Boman, George M. Carlone, Barry S. Fields, Jeannette Guarner, Margaret R. Hammerschlag, Lisa A. Jackson, Cho-Chou Kuo, Matthias Maass, Trudy O. Messmer, Deborah F. Talkington, Maria Lucia Tondella and Sherif R. Zaki

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 33, issue 4, pages 492-503
Published in print August 2001 | ISSN: 1058-4838
Published online August 2001 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/322632
Standardizing Chlamydia pneumoniae Assays: Recommendations from the Centers for Disease Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada)

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Chlamydia pneumoniae has been associated with atherosclerosis and several other chronic diseases, but reports from different laboratories are highly variable and “gold standards” are lacking, which has led to calls for more standardized approaches to diagnostic testing. Using leading researchers in the field, we reviewed the available approaches to serological testing, culture, DNA amplification, and tissue diagnostics to make specific recommendations. With regard to serological testing, only use of microimmunofluorescence is recommended, standardized definitions for “acute infection” and “past exposure” are proposed, and the use of single immunoglobulin (Ig) G titers for determining acute infection and IgA for determining chronic infection are discouraged. Confirmation of a positive culture result requires propagation of the isolate or confirmation by use of polymerase chain reaction (PCR). Four of 18 PCR assays described in published reports met the proposed validation criteria. More consistent use of control antibodies and tissues and improvement in skill at identifying staining artifacts are necessary to avoid false-positive results of immunohistochemical staining. These standards should be applied in future investigations and periodically modified as indicated.

Journal Article.  7368 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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