Journal Article

Use of Real-Time PCR on Blood Samples for Diagnosis of Invasive Aspergillosis

Masahiro Kami, Takafumi Fukui, Seishi Ogawa, Yukimasa Kazuyama, Utako Machida, Yuji Tanaka, Yoshinobu Kanda, Takeshi Kashima, Yasuaki Yamazaki, Tamae Hamaki, Shin-ichiro Mori, Hideki Akiyama, Yoshitomo Mutou, Hisashi Sakamaki, Kazuoki Osumi, Satoshi Kimura and Hisamaru Hirai

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 33, issue 9, pages 1504-1512
Published in print November 2001 | ISSN: 1058-4838
Published online November 2001 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/323337
Use of Real-Time PCR on Blood Samples for Diagnosis of Invasive Aspergillosis

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We developed a new quantitative system for diagnosis of invasive pulmonary aspergillosis (IPA) using real-time automated polymerase chain reaction (PCR). Intra-assay and interassay precision rates for in vitro examination were 2.53% and 2.20%, respectively, and the linearity of this assay was obtained when there were >20 copies/well. We examined 323 samples taken from 122 patients with hematological malignancies, including 33 patients with IPA and 89 control patients. Blood samples were subjected to PCR antigen detection methods, using enzyme-linked immunosorbent assay (ELISA) and determination of plasma (1→3)-β-D-glucan (BDG) concentration. The sensitivities of PCR, ELISA, and BDG measurement for diagnosis of IPA were 79%, 58%, and 67%, respectively; the specificities were 92%, 97%, and 84%. Positive findings on PCR preceded those of computed tomography by -0.3 ± 6.6 days, those of BDG measurement by 6.5 ± 4.9 days, and those of ELISA by 2.8 ± 4.1 days. Real-time PCR was sensitive for IPA diagnosis, and quantitation was accurate.

Journal Article.  4335 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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