Journal Article

Multilaboratory Comparison of Growth Characteristics for Anaerobes, Using 5 Different Agar Media

D. E. Roe, S. M. Finegold, D. M. Citron, E. J. C. Goldstein, H. M. Wexler, J. E. Rosenblatt, M. E. Cox, S. G. Jenkins and D. W. Hecht

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 35, issue Supplement_1, pages S36-S39
Published in print September 2002 | ISSN: 1058-4838
Published online September 2002 | e-ISSN: 1537-6591 | DOI: https://dx.doi.org/10.1086/341918
Multilaboratory Comparison of Growth Characteristics for Anaerobes, Using 5 Different Agar Media

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A multilaboratory study compared the growth of 30 fastidious anaerobes, using 5 different agar media: Wilkins-Chalgren (WC), WC with either whole or laked sheep blood, and Brucella supplemented with vitamin K1 and hemin and either laked or whole sheep blood. The media were compared for quality and quantity of growth. Experiments were conducted either entirely in an anaerobic chamber or inoculated in ambient air with anaerobic incubation. The results showed that (1) any medium plus whole or laked blood was better than unsupplemented WC, (2) whole blood and laked blood additives gave similar results, (3) supplemented Brucella with whole or laked blood was superior to WC and WC with whole or laked blood, and (4) anaerobic and aerobic inoculation with anaerobic incubation gave similar results. Brucella agar supplemented with whole or laked blood supports the growth of fastidious anaerobic species better than the WC agars do.

Journal Article.  1852 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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