Journal Article

Multilaboratory Comparison of Anaerobe Susceptibility Results Using 3 Different Agar Media

D. E. Roe, S. M. Finegold, D. M. Citron, E. J. C. Goldstein, H. M. Wexler, J. E. Rosenblatt, M. E. Cox, S. G. Jenkins and D. W. Hecht

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 35, issue Supplement_1, pages S40-S46
Published in print September 2002 | ISSN: 1058-4838
Published online September 2002 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/341919
Multilaboratory Comparison of Anaerobe Susceptibility Results Using 3 Different Agar Media

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A 5-laboratory study was performed that used the National Committee for Clinical Laboratory Standards (NCCLS) reference agar dilution method with 3 media formulations to determine whether the use of different media would affect minimum inhibitory concentration (MIC) results. Wilkins-Chalgren, Brucella-based blood agar (BRU), and Wilkins-Chalgren agar plus blood (WCB) and 6 antibiotics (clindamycin, cefoxitin, ceftizoxime, piperacillin, metronidazole, and trovafloxacin) were evaluated with 58 isolates. The MIC values were compared, and a significant correlation of>0.80 was demonstrated for all media and each antibiotic/organism group. The cumulative rate of errors for all antibiotics was 0.1%. These data indicate that a change in the NCCLS reference medium for testing of anaerobic bacteria susceptibility to either BRU or WCB will not affect the MIC results for the antibiotics and organisms evaluated.

Journal Article.  3094 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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