Journal Article

Clinical Utility of Polymerase Chain Reaction—Based Detection of <i>Leishmania</i> in the Diagnosis of American Cutaneous Leishmaniasis

Camila Indiani de Oliveira, André Báfica, Fabiano Oliveira, Cecilia B. F. Favali, Tania Correa, Luiz A. R. Freitas, Eliane Nascimento, Jackson M. Costa and Aldina Barral

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 37, issue 11, pages e149-e153
Published in print December 2003 | ISSN: 1058-4838
Published online December 2003 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/379610
Clinical Utility of Polymerase Chain Reaction—Based Detection of Leishmania in the Diagnosis of American Cutaneous Leishmaniasis

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We evaluated the use of polymerase chain reaction (PCR) for diagnosis of American cutaneous leishmaniasis (ACL) in an area in Bahia, Brazil, where Leishmania braziliensis is endemic. Leishmania DNA was detected in 50 cases, yielding a positivity rate of 100%, which was higher than the rates for all of the other diagnostic methods studied—namely, the Montenegro skin test, anti-Leishmania serological testing, and microscopic examination of lesion biopsy specimens. These findings have led us to propose guidelines for the diagnosis of ACL that use PCR as the principal means of parasitological confirmation of cases.

Journal Article.  2950 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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