Journal Article

Mitochondrial DNA Levels in Fat and Blood Cells from Patients with Lipodystrophy or Peripheral Neuropathy and the Effect of 90 Days of High-Dose Coenzyme Q Treatment: A Randomized, Double-Blind, Placebo-Controlled Pilot Study

Eva Rabing Christensen, Marc Stegger, Søren Jensen-Fangel, Alex Lund Laursen and Lars Ostergaard

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 39, issue 9, pages 1371-1379
Published in print November 2004 | ISSN: 1058-4838
Published online November 2004 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/424881
Mitochondrial DNA Levels in Fat and Blood Cells from Patients with Lipodystrophy or Peripheral Neuropathy and the Effect of 90 Days of High-Dose Coenzyme Q Treatment: A Randomized, Double-Blind, Placebo-Controlled Pilot Study

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Background. Mitochondrial toxicity can be induced by reverse-transcriptase inhibitors, and an association between levels of mitochondrial DNA (mtDNA) per cell and lipodystrophy, peripheral neuropathy, and HIV infection per se has been suggested. Studies aimed at increasing the oxidative capacity in HIV-infected patients have been sparse.

Methods. Levels of mtDNA in fat and peripheral blood mononuclear cells (PBMCs) from 25 HIV infected patients and 10 healthy control subjects were studied with real-time PCR analysis. A placebo-controlled and double-blind design was used to assign individuals to receive either 100 mg of coenzyme Q twice daily for 3 months or a matching placebo regimen. Levels of mtDNA and other parameters were assessed before and after the intervention period.

Results. The mean number of mtDNA copies per cell was lower in fat tissue obtained from patients with peripheral neuropathy (1547 mtDNA copies/cell; P = .045), patients with lipodystrophy (1732 mtDNA copies/cell; P = .003) and in HIV patients with no complications associated with highly active antiretroviral therapy (2935 mtDNA copies/cell; P = .078), compared with healthy control subjects (6198 mtDNA copies/cell). No clear difference was seen in mtDNA content in PBMCs. Coenzyme Q therapy improved the general condition of patients (P = .005) and caused a reversible increase in peripheral neuropathy pain (P = .048). Compared with placebo, treatment with coenzyme Q did not result in changes in mtDNA levels in fat cells or in PBMCs after the treatment period.

Conclusions. Levels of mtDNA in fat tissue, but not in PBMCs, were associated with peripheral neuropathy and lipodystrophy. High-dose coenzyme Q therapy increased well-being in asymptomatic HIV-infected patients and those with lipodystrophy, as well as in control subjects, but aggravated pain in patients with peripheral neuropathy.

Journal Article.  4219 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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