Journal Article

Measurement of Immunoglobulin G against Mycobacterial Antigen A60 in Patients with Cystic Fibrosis and Lung Infection Due to <i>Mycobacterium abscessus</i>

Agnès Ferroni, Isabelle Sermet-Gaudelus, Muriel Le Bourgeois, Catherine Pierre-Audigier, Catherine Offredo, Martin Rottman, Didier Guillemot, Claire Bernède, Véronique Vincent, Patrick Berche and Jean-Louis Gaillard

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 40, issue 1, pages 58-66
Published in print January 2005 | ISSN: 1058-4838
Published online January 2005 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/426442
Measurement of Immunoglobulin G against Mycobacterial Antigen A60 in Patients with Cystic Fibrosis and Lung Infection Due to Mycobacterium abscessus

More Like This

Show all results sharing these subjects:

  • Infectious Diseases
  • Immunology
  • Public Health and Epidemiology
  • Microbiology

GO

Show Summary Details

Preview

Background. The diagnosis and prognosis of lung infections due to the emerging nontuberculous mycobacterium (NTM) Mycobacterium abscessus are difficult to establish in children with cystic fibrosis.

Methods. We evaluated the usefulness of an enzyme-linked immunosorbent assay for detecting serum IgG antibodies against the ubiquitous mycobacterial antigen A60.

Results. A total of 186 patients with cystic fibrosis (mean age ± SD, 12.0 ± 5.0 years) were studied, including 15 M. abscessus—positive patients who fulfilled American Thoracic Society (ATS) criteria for NTM infection (M. abscessus—infected patients), 7 M. abscessus—positive patients who did not fulfill ATS criteria, 20 patients positive for various other NTM who did not fulfill ATS criteria, and 144 NTM-negative control patients; mean IgG titers (±SD) for these 4 groups were 718 ± 342 U, 193 ± 113 U, 129 ± 49 U, and 121 ± 53 U, respectively (M. abscessus—infected patients vs. each of the other groups, P < .005). The A60 IgG test was both sensitive (∼87%) and specific (∼95%) if adapted cutoff values were used (150 U and 250 U for patients aged ⩽10 years and patients aged >10 years, respectively) and correlated well with results of acid-fast bacilli smears.

Conclusion. Measurement of anti-A60 IgG may be useful for both the diagnosis and assessment of activity of M. abscessus lung infection in persons with cystic fibrosis.

Journal Article.  4284 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.