Journal Article

Prolonged Incubation and Extensive Subculturing Do Not Increase Recovery of Clinically Significant Microorganisms from Standard Automated Blood Cultures

Ellen Jo Baron, John D. Scott and Lucy S. Tompkins

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 41, issue 11, pages 1677-1680
Published in print December 2005 | ISSN: 1058-4838
Published online December 2005 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/497595
Prolonged Incubation and Extensive Subculturing Do Not Increase Recovery of Clinically Significant Microorganisms from Standard Automated Blood Cultures

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An extensive blood culture protocol, including prolonged incubation of cultures, for 215 patients believed to have had endocarditis yielded only 3 clinically relevant results. Twenty-four Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella (i.e., HACEK) organisms were recovered from standard 5-day blood cultures during the same time period. Specialized methods and not extended incubation times are recommended for recovery of fastidious agents of septicemia.

Journal Article.  2577 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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