Journal Article

Intragenic Variability of Human Cytomegalovirus Glycoprotein B in Clinical Strains

Ursula Meyer-König, Michael Haberland, Dorothee von Laer, Otto Haller and Frank T. Hufert

in The Journal of Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 177, issue 5, pages 1162-1169
Published in print May 1998 | ISSN: 0022-1899
Published online May 1998 | e-ISSN: 1537-6613 | DOI: https://dx.doi.org/10.1086/515262
Intragenic Variability of Human Cytomegalovirus Glycoprotein B in Clinical Strains

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Human cytomegalovirus (HCMV) strains can be classified into four glycoprotein B (gB) genotypes, and there has been evidence of differences in viral virulence. In this study, intragenic variability of HCMV gB strains was analyzed. The gB gene was amplified by nested polymerase chain reaction using samples from immunosuppressed patients. The genotype of fragments corresponding to the cleavage site of gB was determined by restriction fragment analysis; fragments corresponding to the N- and C-termini (gBn and gBc) were sequenced and compared with published sequences. At the cleavage site, the four known genotypes were found. Typing revealed four major genotypes at the N-terminus and two at the C-terminus. In 22 of 44 strains, the gB type determined at the cleavage site was different from the gBn or gBc type (or either), indicating that intragenic variability within the gB gene occurs frequently.

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Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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