Journal Article

Bilophila <i>wadsworthia</i> Clinical Isolates Compared by Polymerase Chain Reaction Fingerprinting

S. Hunt Gerardo, M. Marina, D. M. Citron, M. C. Claros, M. K. Hudspeth and E. J. C. Goldstein

in Clinical Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 25, issue Supplement_2, pages S291-S294
Published in print September 1997 | ISSN: 1058-4838
Published online September 1997 | e-ISSN: 1537-6591 | DOI: http://dx.doi.org/10.1086/516229
Bilophila wadsworthia Clinical Isolates Compared by Polymerase Chain Reaction Fingerprinting

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Bilophila wadsworthia isolates recovered from a right-ear cholesteatoma and brain abscess of the same patient were analyzed by means of polymerase chain reaction (PCR) fingerprinting with single primers (T3B and M13 core) to ascertain if they originated from the same clone. Their PCR fingerprint profiles were compared with those of three additional B. wadsworthia clinical isolates and the type strain (ATCC 49260). The two isolates from the same patient produced PCR fingerprint profiles identical to each other, regardless of which primer was used. All isolates' PCR fingerprint profiles, with use of either the T3B or M13 core primer, shared some major and minor bands. However, differences in additional major and minor bands distinguished each of the additional isolates, suggesting that there are different subgroups of B. wadsworthia.

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Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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