Chapter

Single cell techniques

Alison M. Gurney

in The Pharmacology of Vascular Smooth Muscle

Published in print February 1996 | ISBN: 9780192623874
Published online March 2012 | e-ISBN: 9780191724671 | DOI: http://dx.doi.org/10.1093/acprof:oso/9780192623874.003.0003
Single cell techniques

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By isolating single cells from the smooth muscle or endothelial layer of a blood vessel, it is possible to study signal transduction mechanisms in these cells without the need to worry about indirect effects resulting from actions on other cell types. Isolated cells also have the advantage of being more amenable to experimental techniques that can directly address questions relating to receptor mechanisms and intracellular pathways. This chapter reviews methods for isolating vascular cells and outlines techniques that are particularly well suited to single cell studies, namely voltage- and patch-clamp recording, measurement of intracellular ion concentrations, and photolysis of caged compounds. These techniques have been successfully applied to the study of endothelial and smooth muscle cell function. The aim here is to discuss the information that each technique can provide, along with their advantages and disadvantages.

Keywords: smooth muscle; endothelial layer; signal transduction mechanisms; isolated cells; intracellular pathways; photolysis

Chapter.  12983 words.  Illustrated.

Subjects: Neuroscience

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