First analysis of macromolecular crystals

Sherin S. Abdel-Meguid, David Jeruzalmi and Mark R. Sanderson

in Macromolecular Crystallography

Published in print August 2007 | ISBN: 9780198520979
Published online September 2007 | e-ISBN: 9780191706295 | DOI:
 First analysis of macromolecular crystals

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The past decade and a half have witnessed an almost complete revolution in the way that macromolecular diffraction data are recorded. The promise of diffraction data measurements essentially free of the effects of radiation damage has driven a change from older methods requiring crystalline samples to be mounted in thin glass capillaries for measurements at ambient temperatures (or so) to newer, experimental schemes that enable measurements at cryogenic temperatures from crystals mounted in free-standing films. Preparation of macromolecular crystalline samples for measurements at cryogenic temperatures has three separable: cryoprotection, shock-cooling, and cryogenic transfer to the X-ray diffraction camera. This chapter describes each of these in detail.

Keywords: macromolecular diffraction; macromolecular crystals; X-ray diffraction; cryoprotection; shock-cooling; cryogenic transfer

Chapter.  7424 words.  Illustrated.

Subjects: Biochemistry

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