Chapter

HREM IN BIOLOGY, ORGANIC CRYSTALS, AND RADIATION DAMAGE

JOHN C. H. SPENCE

in High-Resolution Electron Microscopy

Third edition

Published in print October 2008 | ISBN: 9780199552757
Published online January 2010 | e-ISBN: 9780191708664 | DOI: http://dx.doi.org/10.1093/acprof:oso/9780199552757.003.0006

Series: Monographs on the Physics and Chemistry of Materials

 HREM IN BIOLOGY, ORGANIC CRYSTALS, AND RADIATION DAMAGE

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This chapter discusses the relationship between ‘absorption’ and phase contrast for biological imaging. It then discusses the theory for imaging single atoms, with full detail of an experimental example. The optimum accelerating voltage is discussed, and the dependence of bright field image contrast on atomic number reviewed. Dark field and elastically filtered modes are reviewed. Noise, information, the Rose equation, electron tomography, and the imaging of two-dimensional protein crystals are discussed in cryo-electron microscopy. Sections on radiation damage mechanisms and imaging organic crystals are included.

Keywords: cryo-electron microscopy; Rose equation; radiation damage; single-particle imaging; two-dimensional crystals; elastic filtering; ionization damage; single-atom imaging

Chapter.  20662 words.  Illustrated.

Subjects: Atomic, Molecular, and Optical Physics

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