Chapter

3D electron crystallography

Xiaodong Zou, Sven Hovmöller and Peter Oleynikov

in Electron Crystallography

Published in print August 2011 | ISBN: 9780199580200
Published online January 2012 | e-ISBN: 9780191731211 | DOI: http://dx.doi.org/10.1093/acprof:oso/9780199580200.003.0011

Series: International Union of Crystallography Texts on Crystallography

3D electron crystallography

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All structures are 3-dimensional, but each EM image is just a 2D projection of the 3D structure. Until recently, almost all work in the EM field has been 2D. 3D electron crystallography can be made by combining several HRTEM images and/or ED patterns taken from different orientations (zone axes). A crystal with all unit-cell dimensions over 5 Å will never give rise to projections that can be interpreted in terms of atomic coordinates. A 3D structure is needed. The procedure from image collection and interpretation, in terms of zone axis and projected symmetry, to combination of data into a 3D map, is demonstrated. All the steps are followed in great detail, using a very complex intermetallic structure as an example. HRTEM images (before and after image processing) and ED patterns from several zone axes are show together with a table of amplitudes and phases extracted from the HRTEM images and ED patterns. The remarkably clear 3D potential map is interpreted in terms of atomic structure.

Keywords: 3D electron crystallography; 3D reconstruction; high-resolution electron microscopy; electron diffraction; combining 2D projections; zone axes; merging projections; intermetallic compounds; 3D potential map; 3D atomic coordinates; contrast transfer function; symmetry; origin determination

Chapter.  10280 words.  Illustrated.

Subjects: Crystallography

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