Journal Article

Characterization of an EPG Waveform Library for Adult <i>Lygus lineolaris</i> and <i>Lygus hesperus</i> (Hemiptera: Miridae) Feeding on Cotton Squares

Felix A. Cervantes, Elaine A. Backus, Larry Godfrey, Waseem Akbar and Thomas L. Clark

in Annals of the Entomological Society of America

Published on behalf of Entomological Society of America

Volume 109, issue 5, pages 684-697
Published in print September 2016 | ISSN: 0013-8746
Published online June 2016 | e-ISSN: 1938-2901 | DOI: https://dx.doi.org/10.1093/aesa/saw039
Characterization of an EPG Waveform Library for Adult Lygus lineolaris and Lygus hesperus (Hemiptera: Miridae) Feeding on Cotton Squares

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Lygus lineolaris (Palisot de Beauvois) and Lygus hesperus Knight are among the most important pests affecting production of cotton in the United States. Lygus spp. use a cell rupture feeding strategy. However, the precise stylet-probing behaviors of adult Lygus spp. are not well understood or quantifiably related to cotton damage. The long-term goals of our research are to: 1) relate stylet probing to damage, and 2) quantitatively compare L. lineolaris feeding among resistant and susceptible host plants. The specific objectives of this study were to apply the latest technology in electropenetrography (EPG) to record adult, prereproductive Lygus spp. feeding, to identify and characterize all the waveforms, and to hypothesize their biological meanings. We used a third-generation AC–DC electropenetrograph to record nonprobing and probing waveforms of adult bugs on pinhead (<3 mm) cotton squares, and constructed a waveform library from the output. Recordings were obtained with both AC and DC applied signals and at different input impedances (106–109 Ω). Three nonprobing waveforms were identified and visually correlated: Standing still (S), walking (W), and antennation (A). Probing waveforms were classified as: cell rupturing (CR), transition (T), and ingestion (I). T waveform is the first finding of an X wave for a nonsalivary sheath feeder in Hemiptera, implying that tasting/testing/acceptance behaviors can be performed by a cell rupture feeder. While waveform I is not performed in every probe, when performed, its appearance and structure were affected by applied signal and input impedance. Overall, time spent in nonprobing behaviors was longer than time spent on probing behaviors.

Keywords: feeding behavior; input impedance; applied signal; stylet penetration; electrical penetration graph

Journal Article.  9274 words.  Illustrated.

Subjects: Entomology

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