Journal Article

Simultaneous Determination of γ-Hydroxybutyrate (GHB) and its Analogues (GBL, 1.4-BD, GVL) in Whole Blood and Urine by Liquid Chromatography Coupled to Tandem Mass Spectrometry

Sys Stybe Johansen and Charlotte Norup Windberg

in Journal of Analytical Toxicology

Volume 35, issue 1, pages 8-14
Published in print January 2011 | ISSN: 0146-4760
Published online January 2011 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/anatox/35.1.8
Simultaneous Determination of γ-Hydroxybutyrate (GHB) and its Analogues (GBL, 1.4-BD, GVL) in Whole Blood and Urine by Liquid Chromatography Coupled to Tandem Mass Spectrometry

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A simple liquid chromatography-tandem mass spectrometry (LC-MS-MS) method has been developed and validated for simultaneous identification and quantification of γ-hydroxybutyrate (GHB), γ-butyrolactone (GBL), 1.4-butanediol (1.4-BD), and γ-valerolactone (GVL) in whole blood from forensic cases. The sample preparation of whole blood involved protein precipitation by acidic methanol. Urine samples were diluted and evaluated in relation to a control at the cutoff concentration. Hexadeutero GHB (GHB-d6) was used as the internal standard. Separation was achieved by reversed-phase chromatography, and detection was by MS-MS in MRM mode. The linear range for all compounds was from 1.0 to 100 mg/kg in whole blood with a limit of quantification of about 1 mg/kg. The method was validated with regards to selectivity, recovery, accuracy and precision, and stability. The method is currently applied to investigations on suspected drug-facilitated sexual assaults, driving under the influence of drugs, and general intoxication with these substances.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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