Journal Article

A Rapid and Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Olopatadine Concentration in Human Plasma

Ping Zhu, Yu-Guan Wen, Xiao-Ping Fan, Zhi-Ling Zhou, Rui-Xin Fan, Ji-Mei Chen, Ke-li Huang, Xiao-lan Zhu, Yan-Fang Chen and Jian Zhuang

in Journal of Analytical Toxicology

Volume 35, issue 2, pages 113-118
Published in print March 2011 | ISSN: 0146-4760
Published online March 2011 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/anatox/35.2.113
A Rapid and Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for Determination of Olopatadine Concentration in Human Plasma

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A sensitive and rapid method based on liquid chromatography-tandem mass spectrometry (MS-MS) was developed for the determination of olopatadine in human plasma. Sample preparations were carried out by protein precipitation with the addition of acetonitrile followed by liquid-liquid extraction with ethyl acetate/dichloromethane after internal standard (IS, amitriptyline) spiked. After evaporation to dryness, the resultant residue was reconstituted in mobile phase. Separation of olopatadine and IS from the interferences was achieved on a C18 column followed by MS-MS detection. The analytes were monitored in the positive ionization mode with a TurboIonspray source. Multiple reaction monitoring using the transition of m/z 338 → 165 and m/z 278 → 91 was performed to quantify olopatadine and IS, respectively. The method had a total chromatographic run time of 3.5 min and linear calibration curves over the concentration range of 0.2–100 ng/mL. The lower limit of quantification was 0.2 ng/mL. For each QC concentration level the intra- and interday precisions were less than 11.4%, and relative errors ranged between −6.40% and 9.26%. The validated method was successfully applied to the quantification of olopatadine concentration in human plasma after administration of olopatadine at an oral dose of 5 mg in order to evaluate the pharmacokinetics.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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