Journal Article

Determination of Platinum Originating from Carboplatin in Human Urine and Canine Excretion Products by Inductively Coupled Plasma Mass Spectrometry

Tine Janssens, Elke E.M. Brouwers, Johan P. de Vos, Jan H.M. Schellens and Jos H. Beijnen

in Journal of Analytical Toxicology

Volume 35, issue 3, pages 153-161
Published in print April 2011 | ISSN: 0146-4760
Published online April 2011 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/anatox/35.3.153
Determination of Platinum Originating from Carboplatin in Human Urine and Canine Excretion Products by Inductively Coupled Plasma Mass Spectrometry

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We present highly sensitive, rapid methods for the determination of Pt originating from carboplatin in human urine and canine urine, feces, and oral fluid. The methods are based on the quantification of Pt by inductively coupled plasma mass spectrometry, and allow quantification of 7.50 ng/L Pt in human and canine urine (in 15 µL of matrix), 15.0 ng/L Pt in canine oral fluid (in 15 µL of matrix), and 0.105 ng/g Pt in canine feces (in 5 µg of matrix). Sample pretreatment mainly involved dilution with appropriate diluents. The performance of the methods fulfilled the most recent FDA guidelines for bioanalytical method validation. Validated ranges of quantification were 7.50 to 1.00 × 104 ng/L Pt in human and canine urine, 0.105–30.0 ng/g Pt in canine feces, and 15.0 to 1.00 × 104 ng/L Pt in canine oral fluid. Canine urine and oral fluid cannot be easily obtained. Therefore, we also investigated the validity of the usage of human matrix samples for the preparation of calibration standards and quality control samples as alternatives, to be used in future clinical studies. The assays are used to support biomonitoring studies and pharmacokinetic studies in pet dogs treated with carboplatin.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

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