Journal Article

Novel Method for Simultaneous Aqueous in Situ Derivatization of THC and THC-COOH in Human Urine Samples: Validation and Application to Real Samples

S. Chericoni, I. Battistini, S. Dugheri, M. Pacenti and M. Giusiani

in Journal of Analytical Toxicology

Volume 35, issue 4, pages 193-198
Published in print May 2011 | ISSN: 0146-4760
Published online May 2011 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/anatox/35.4.193
Novel Method for Simultaneous Aqueous in Situ Derivatization of THC and THC-COOH in Human Urine Samples: Validation and Application to Real Samples

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The present work describes the validation of a novel aqueous in situ derivatization procedure with trimethyloxonium tetrafluoroborate (TMO) as methylating agent for the simultaneous, quantitative analysis of Δ9-tetrahydrocannabinol (THC) and 11-nor-Δ9-tetrahydrocannabinol carboxylic acid (THC-COOH) in human urine. The derivatizing agent is directly added to the urine sample and the methyl-derivatives are then recovered by liquid-liquid extraction procedure. Gas chromatography-mass spectrometry was used to detect the derivatives in selected ion monitoring mode. The limits of detection were 0.7 ng/mL for THC and 0.5 ng/mL for THC-COOH, whereas the limits of quantification were 1.9 and 0.9 ng/mL, respectively. The method has been applied to 60 real samples both positive and negative to immunochemical screening test resulting to be very useful and reliable in routine analysis of THC-COOH in human urine for toxicological and forensic purposes.

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Subjects: Medical Toxicology ; Toxicology (Non-medical)

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