Journal Article

Characterization of the Disposition of Melamine in Female Sprague-Dawley Rats Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

Desheng Wu, Jianjun Liu, Qionghui Zhao, Xinyun Xu, Linqing Yang, Haiyan Huang, Jianhui Yuan, Li Zhou and Zhixiong Zhuang

in Journal of Analytical Toxicology

Volume 35, issue 8, pages 551-557
Published in print October 2011 | ISSN: 0146-4760
Published online October 2011 | e-ISSN: 1945-2403 | DOI: http://dx.doi.org/10.1093/anatox/35.8.551
Characterization of the Disposition of Melamine in Female Sprague-Dawley Rats Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

More Like This

Show all results sharing these subjects:

  • Medical Toxicology
  • Toxicology (Non-medical)

GO

Show Summary Details

Preview

This study characterized the concentration-time profile of melamine in the heart, liver, spleen, lungs, kidneys, bladder, feces, urine, and plasma after melamine (MM) administration. Female Sprague-Dawley rats received a single oral dose of 1.0 g/kg body weight. Samples (n = 4 per time point) were collected at 12, 24, 48, 72, 96, 120, 144, and 168 h. Based on calculations of the area under the concentration-time curves after dosing, ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) was used to detect MM concentration in tissues. Peak concentrations of MM are reached in the livers and lungs at 12 h after dosing and in hearts, spleens, kidneys, bladders, feces, urine, and plasma at 24 h after dosing. More than 90% of the ingested MM is excreted in feces and urine within 24 h. These results provided initial understanding of the tissue disposition of MM. Moreover, this study demonstrates that UPLC-MS-MS can be used to detect MM in biological samples.

Journal Article.  0 words. 

Subjects: Medical Toxicology ; Toxicology (Non-medical)

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.