Journal Article

Vegetative Storage Protein in <i>Litchi chinensis</i>, a Subtropical Evergreen Fruit Tree, Possesses Trypsin Inhibitor Activity

Wei-Min Tian, Shi-Qing Peng, Xu-Chu Wang, Min-Jing Shi, Yue-Yi Chen and Zheng-Hai Hu

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 100, issue 6, pages 1199-1208
Published in print November 2007 | ISSN: 0305-7364
Published online October 2007 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcm216
Vegetative Storage Protein in Litchi chinensis, a Subtropical Evergreen Fruit Tree, Possesses Trypsin Inhibitor Activity

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background and Aims

Vegetative storage proteins (VSPs) are commonly bioactive in herbaceous plants but few VSPs with bioactivity have been identified in trees. In addition, information on the characterization of VSPs in evergreen trees is limited. The objective of this study was to characterize the VSPs with bioactivity in evergreen trees.

Methods

The VSP in lychee (Litchi chinensis), an evergreen fruit tree, was characterized by a combination of cytological, biochemical and molecular biological techniques.

Key Results

The VSP in lychee was a 22-kDa protein. It accumulated in the large central vacuoles of protein-storing cells (PSCs) in two distinguishable forms, granular and floccular. The PSCs were of a novel type. The 22-kDa protein is distributed in mature leaves, bark tissues of branches, trunk and large roots, paralleling the distribution of PSCs. Its homologues were present in mature seed. During young shoot development and fruiting, the 22-kDa protein decreased apparently, suggesting a nitrogen-storage function. The 22-kDa protein had several isoforms encoded by a small multigene family. One gene member, LcVSP1, was cloned. The LcVSP1 had no intron and contained a 675 bp open reading frame encoding a putative protein of 225 amino acids. LcVSP1 was homologous to Kunitz trypsin inhibitors. The 22-kDa protein inhibited trypsin and chymotrypsin, but had no inhibitory effect on subtilisin.

Conclusions

Lychee is rich in a 22-kDa VSP with trypsin inhibitor activity. The VSP plays an important role in nitrogen storage while its possible defensive function remains to be elucidated.

Keywords: Defence; evergreen tree; Kunitz trypsin inhibitor; Litchi chinensis; nitrogen storage; vegetative storage protein

Journal Article.  5614 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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