Journal Article

Isolation and Characterization of Arbuscules from Roots of an Increased-arbuscule-forming Mutant of <i>Lotus japonicus</i>

Keishi Senoo, Zakaria Solaiman, Satoki Tanaka, Masayoshi Kawaguchi, Haruko Imaizumi-Anraku, Shoichiro Akao, Akiyoshi Tanaka and Hitoshi Obata

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 100, issue 7, pages 1599-1603
Published in print December 2007 | ISSN: 0305-7364
Published online October 2007 | e-ISSN: 1095-8290 | DOI: https://dx.doi.org/10.1093/aob/mcm243
Isolation and Characterization of Arbuscules from Roots of an Increased-arbuscule-forming Mutant of Lotus japonicus

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background and Aims

Previous methods for isolation of arbuscules from mycorrhizal roots are time-consuming, complex and expensive. Therefore, a simple, rapid and inexpensive method for the isolation of metabolically active arbuscules from plant root of an increased-arbuscule-forming mutant of Lotus japonicus (Ljsym78-2) is described.

Methods

Roots of the L. japonicus mutant plants Ljsym78-2 colonized by Glomus sp. were separated from soil, washed with water, immersed in CaSO4 before being cut into 5-mm pieces and homogenized with a Waring blender at 6000 rpm for 30 s. The arbuscules were purified by separation from plant tissues with a 50-μm nylon mesh, finally collecting on a 30-μm nylon mesh. Enzyme histochemical staining showed that the collected arbuscules had succinate dehydrogenase, alkaline phosphatase and acid phosphatase activities.

Key Results and Conclusions

The enzymic activity of the arbuscules was not affected after the isolation process. The establishment of this simple, rapid and inexpensive method for the isolation of metabolically active arbuscules will be useful to clarify the biochemical processes occurring in nutrient exchange at the arbuscular interface.

Keywords: Arbuscular mycorrhiza; arbuscule isolation; Glomus sp., increased-arbuscule-forming mutant; Lotus japonicus

Journal Article.  2960 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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