Journal Article

Development of a Genomic Microsatellite Library in Perennial Ryegrass (<i>Lolium perenne</i>) and its Use in Trait Mapping

J. King, D. Thorogood, K. J. Edwards, I. P. Armstead, L. Roberts, K. Skøt, Z. Hanley and I. P. King

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 101, issue 6, pages 845-853
Published in print April 2008 | ISSN: 0305-7364
Published online February 2008 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcn016
Development of a Genomic Microsatellite Library in Perennial Ryegrass (Lolium perenne) and its Use in Trait Mapping

More Like This

Show all results sharing these subjects:

  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Background and Aims

Perennial ryegrass (Lolium perenne) is one of the key forage and amenity grasses throughout the world. In the UK it accounts for 70 % of all agricultural land use with an estimated farm gate value of £6 billion per annum. However, in terms of the genetic resources available, L. perenne has lagged behind other major crops in Poaceae. The aim of this project was therefore the construction of a microsatellite-enriched genomic library for L. perenne to increase the number of genetic markers available for both marker-assisted selection in breeding programmes and gene isolation.

Methods

Primers for 229 non-redundant microsatellite markers were designed and used to screen two L. perenne genotypes, one amenity and one forage. Of the 229 microsatellites, 95 were found to show polymorphism between amenity and forage genotypes. A selection of microsatellite primers was selected from these 95 and used to screen two mapping populations derived from intercrossing and backcrossing the two forage and amenity grass genotypes.

Key Results and Conclusions

The utility of the resulting genetic maps for analysis of the genetic control of target traits was demonstrated by the mapping of genes associated with heading date to linkage groups 4 and 7.

Keywords: Microsatellites; Lolium perenne; perennial ryegrass; trait mapping

Journal Article.  4636 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.