Journal Article

β-1,3 : 1,4-Glucan Synthase Activity in Rice Seedlings under Water

Tomoya Kimpara, Tsutomu Aohara, Kouichi Soga, Kazuyuki Wakabayashi, Takayuki Hoson, Yoichi Tsumuraya and Toshihisa Kotake

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 102, issue 2, pages 221-226
Published in print August 2008 | ISSN: 0305-7364
Published online May 2008 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcn077
β-1,3 : 1,4-Glucan Synthase Activity in Rice Seedlings under Water

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background and Aims

The metabolism of β-1,3 : 1,4-glucan regulates the mechanical properties of cell walls, and thereby changes the elongation growth of Poaceae plants. A previous study has shown that elongation growth of rice coleoptiles under water is enhanced by increased activity of β-1,3 : 1,4-glucan hydrolases; however, the involvement of β-1,3 : 1,4-glucan synthase activity in elongation growth under water has not yet been clarified.

Methods

The β-1,3 : 1,4-glucan synthase activity in a microsomal fraction prepared from rice seedlings grown under water was compared with that from control seedlings grown in air. The change under water in the relative expression level of CslF6, a major isoform of the β-1,3 : 1,4-glucan synthase genes, was examined by quantitative reverse-transcriptase PCR.

Key Results

The level of β-1,3 : 1,4-glucan synthase activity in submerged seedlings decreased to less than 40 % of that of the control seedlings and was accompanied by a significant reduction in the amount of β-1,3 : 1,4-glucan in the cell walls. Under water, the expression of CslF6 was reduced to less than 20 % of the unsubmerged control. Bubble aeration partially restored both β-1,3 : 1,4-glucan synthase activity and the expression of CslF6 under water, correlating with suppression of the submergence-induced elongation growth of coleoptiles.

Conclusions

Submergence down-regulates the expression of the CslF6 gene, leading to a decreased level of β-1,3 : 1,4-glucan synthase activity. Together with the increased activity of β-1,3 : 1,4-glucan hydrolases, the decreased activity of β-1,3 : 1,4-glucan synthase contributes to the decrease in the amount of β-1,3 : 1,4-glucan in the cell walls under water. The suppression of β-1,3 : 1,4-glucan synthesis under water may be mainly due to oxygen depletion.

Keywords: β-1,3 : 1,4-glucan; rice; Oryza sativa; elongation growth; cell wall; β-1,3 : 1,4-glucan synthase; CslF gene

Journal Article.  4168 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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