Journal Article

Genetic Variation of Stomatal Traits and Carbon Isotope Discrimination in Two Hybrid Poplar Families (<i>Populus deltoides</i> ‘S9-2’ × <i>P. nigra</i> ‘Ghoy’ and <i>P. deltoides</i> ‘S9-2’ × <i>P. trichocarpa</i> ‘V24’)

Sophie Y. Dillen, Nicolas Marron, Barbra Koch and Reinhart Ceulemans

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 102, issue 3, pages 399-407
Published in print September 2008 | ISSN: 0305-7364
Published online June 2008 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcn107
Genetic Variation of Stomatal Traits and Carbon Isotope Discrimination in Two Hybrid Poplar Families (Populus deltoides ‘S9-2’ × P. nigra ‘Ghoy’ and P. deltoides ‘S9-2’ × P. trichocarpa ‘V24’)

More Like This

Show all results sharing these subjects:

  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Background and Aims

Stomata play an important role in both the CO2 assimilation and water relations of trees. Therefore, stomatal traits have been suggested as criteria for selection of clones or genotypes which are more productive and have larger water-use efficiency (WUE) than others. However, the relationships between plant growth, WUE and stomatal traits are still unclear depending on plant material (genus, species, families, genotypes) and, more precisely, on the strength of the relationships between the plants. In this study, the correlations between these three traits categories, i.e. plant growth, WUE and stomatal traits, were compared in two related poplar families.

Methods

Stomatal traits (stomatal density, length and ratio adaxial : abaxial stomatal densities) of a selection of F1 genotypes and the parents of two hybrid poplar families Populus deltoides ‘S9-2’ × P. nigra ‘Ghoy’ (D × N family, 50 F1) and P. deltoides ‘S9-2’ × P. trichocarpa ‘V24’ (D × T family, 50 F1) were measured, together with stem height and circumference. Carbon isotope discrimination (Δ) was determined and used as an indicator of leaf-level intrinsic WUE.

Key Results

Leaves of hybrids and parents were amphistomatous, except for the P. trichocarpa parent. Both families displayed high values of heritability for stomatal traits and Δ. In the progeny, the relationship between stem circumference and Δ was weak for the D × N family, while abaxial and total stomatal density were positively associated with stem dimensions for the D × T family only.

Conclusions

Genetic variation in stomatal traits and Δ was large within as well as between the different poplar species and their hybrids, but there were no direct relationships between stomatal traits and plant growth or Δ. As already noticed in various poplar hybrids, the absence of, or the weak, relationship between Δ and plant growth allows the possibility of selecting poplar genotypes combining high productivity and high WUE. In this study, stomatal traits are of limited value as criteria for selection of genotypes with good growth and large WUE.

Keywords: Adaxial and abaxial stomatal density; stomatal length; heritability; water-use efficiency (WUE); F1 hybrids; breeding; Populus deltoides; Populus nigra; Populus trichocarpa

Journal Article.  6045 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.