Journal Article

Impact of Iron Supply on the Kinetics of Recovery of Photosynthesis in Cd-stressed Poplar (<i>Populus glauca</i>)

Ádám Solti, László Gáspár, Ilona Mészáros, Zoltán Szigeti, László Lévai and Éva Sárvári

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 102, issue 5, pages 771-782
Published in print November 2008 | ISSN: 0305-7364
Published online August 2008 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcn160
Impact of Iron Supply on the Kinetics of Recovery of Photosynthesis in Cd-stressed Poplar (Populus glauca)

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Background and Aims

Cadmium (Cd) causes Fe-deficiency-like symptoms in plants, and strongly inhibits photosynthesis. To clarify the importance of Cd-induced Fe deficiency in Cd effects on photosynthesis, the recovery processes were studied by supplying excess Fe after the Cd symptoms had developed.

Methods

Fe-citrate at 10 µm or 50 µm was given with or without 10 µm Cd(NO3)2 to hydroponically cultured poplars (Populus glauca ‘Kopeczkii’) with characteristic Cd symptoms. Ion, chlorophyll and pigment contents, amount of photosynthetic pigment–protein complexes, chlorophyll fluorescence and carbon assimilation were measured together with the mapping of healing processes by fluorescence imaging.

Key Results

In regenerated leaves, the iron content increased significantly, while the Cd content did not decrease. As a result, the structural (increase in the amount of photosynthetic pigments and pigment–protein complexes, decrease in the F690/F740 ratio) and functional (elevation of CO2 fixation activity and ΔF/Fm′) recovery of the photosynthetic machinery was detected. Cd-induced, light-stress-related changes in non-photochemical quenching, activity of the xanthophyll cycle, and the F440?/F520 ratio were also normalized. Imaging the changes in chlorophyll fluorescence, the recovery started from the parts adjacent to the veins and gradually extended to the interveinal parts. Kinetically, the rate of recovery depended greatly on the extent of the Fe supply, and chlorophyll a/b ratio and ΔF/Fm′ proved to be the most-rapidly reacting parameters.

Conclusions

Iron deficiency is a key factor in Cd-induced inhibition of photosynthesis.

Keywords: Cadmium; chlorophyll–protein; iron deficiency; poplar; Populus glauca Haines 1906 var. Kopeczkii; fluorescence imaging; chlorophyll fluorescence induction

Journal Article.  7135 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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