Journal Article

Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome

Longbiao Guo, Chin-Min Kimmy Ho, Zhaosheng Kong, Yuh-Ru Julie Lee, Qian Qian and Bo Liu

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 103, issue 3, pages 387-402
Published in print February 2009 | ISSN: 0305-7364
Published online December 2008 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcn248
Evaluating the microtubule cytoskeleton and its interacting proteins in monocots by mining the rice genome

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  • Ecology and Conservation
  • Evolutionary Biology
  • Plant Sciences and Forestry

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Background

Microtubules (MTs) are assembled by heterodimers of α- and β-tubulins, which provide tracks for directional transport and frameworks for the spindle apparatus and the phragmoplast. MT nucleation and dynamics are regulated by components such as the γ-tubulin complex which are conserved among eukaryotes, and other components which are unique to plants. Following remarkable progress made in the model plant Arabidopsis thaliana toward revealing key components regulating MT activities, the completed rice (Oryza sativa) genome has prompted a survey of the MT cytoskeleton in this important crop as a model for monocots.

Scope

The rice genome contains three α-tubulin genes, eight β-tubulin genes and a single γ-tubulin gene. A functional γ-tubulin ring complex is expected to form in rice as genes encoding all components of the complex are present. Among proteins that interact with MTs, compared with A. thaliana, rice has more genes encoding some members such as the MAP65/Ase1p/PRC1 family, but fewer for the motor kinesins, the end-binding protein EB1 and the mitotic kinase Aurora. Although most known MT-interacting factors have apparent orthologues in rice, no orthologues of arabidopsis RIC1 and MAP18 have been identified in rice. Among all proteins surveyed here, only a few have had their functions characterized by genetic means in rice. Elucidating functions of proteins of the rice MT cytoskeleton, aided by recent technical advances made in this model monocot, will greatly advance our knowledge of how monocots employ their MTs to regulate their growth and form.

Keywords: Cytoskeleton; kinesins; microtubules (MTs); microtubule-associated proteins (MAPs); motors; rice; Oryza sativa

Journal Article.  11204 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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