Journal Article

Novel localization of callose in the spores of <i>Physcomitrella patens</i> and phylogenomics of the callose synthase gene family

Scott Schuette, Andrew J. Wood, Matt Geisler, Jane Geisler-Lee, Roberto Ligrone and Karen S. Renzaglia

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 103, issue 5, pages 749-756
Published in print March 2009 | ISSN: 0305-7364
Published online January 2009 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcn268
Novel localization of callose in the spores of Physcomitrella patens and phylogenomics of the callose synthase gene family

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  • Ecology and Conservation
  • Evolutionary Biology
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Background and Aims

Callose involvement in spore development is a plesiomorphic feature of land plants. Correlated light, fluorescence and immuno-electron microscopy was conducted on the developing spores of Physcomitrella patens to probe for callose. Using a bioinformatic approach, the callose synthase (PpCalS) genes were annotated and PpCalS and AtCalS gene families compared, testing the hypothesis that an exine development orthologue is present in P. patens based on deduced polypeptide similarity with AtCalS5, a known exine development gene.

Methods

Spores were stained with aniline blue fluorescent dye. Capsules were prepared for immuno-light and immuno-electron microscopy by gold labelling callose epitopes with monoclonal antibody. BLAST searches were conducted using the AtCalS5 sequence as a query against the P. patens genome. Phylogenomic analysis of the CalS gene family was conducted using PAUP (v.4·1b10).

Key Results

Callose is briefly present in the aperture of developing P. patens spores. The PpCalS gene family consists of 12 copies that fall into three distinct clades with AtCalS genes. PpCalS5 is an orthologue to AtCalS5 with highly conserved domains and 64 % similarity of their deduced polypeptides.

Conclusions

This is the first study to identify the presence of callose in moss spores. AtCalS5 was previously shown to be involved in pollen exine development, thus making PpCalS5 a suspect gene involved in moss spore exine development.

Keywords: Bryophyte; callose; callose synthase; exine development; moss; Physcomitrella patens; spores; sporogenesis

Journal Article.  4585 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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