Journal Article

Proteomic identification of small, copper-responsive proteins in germinating embryos of <i>Oryza sativa</i>

Hongxiao Zhang, Chunlan Lian and Zhenguo Shen

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 103, issue 6, pages 923-930
Published in print April 2009 | ISSN: 0305-7364
Published online February 2009 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcp012
Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa

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  • Ecology and Conservation
  • Evolutionary Biology
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Background and Aims

Although copper (Cu) is an essential micronutrient for plants and algae, excess Cu is toxic to most plants and can cause a wide range of deleterious effects. To investigate the response of rice (Oryza sativa) to Cu stress, a proteomic approach was used to analyse Cu stress-induced changes in the expression of low molecular-weight proteins in germinating rice seed embryos.

Methods

Rice seeds were germinated in the presence or absence of 200 µm Cu for 6 d, and embryos, including newly formed shoots and radicles, were isolated. After proteins were extracted from the germinating embryos and separated by two-dimensional PAGE, 16 proteins in the 6- to 25-kDa range were identified using MALDI-TOF mass spectrometry.

Key Results and Conclusions

Thirteen of the proteins identified, including metallothionein-like protein, membrane-associated protein-like protein, putative wall-associated protein kinase, pathogenesis-related proteins and the putative small GTP-binding protein Rab2, were up-regulated by Cu stress. Three proteins, a putative small cytochrome P450 (CYP90D2), a putative thioredoxin and a putative GTPase, were down-regulated by Cu stress. As far as is known, this study provides the first proteomic evidence that metallothionein and CYP90D2 are Cu-responsive proteins in plants. These findings may lead to a better understanding of plant molecular responses to toxic metal exposure.

Keywords: Copper; metallothionein; rice; Oryza sativa; proteomics; seed germination

Journal Article.  5630 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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