Journal Article

<i>Arabidopsis</i> plants harbouring a mutation in <i>AtSUC2</i>, encoding the predominant sucrose/proton symporter necessary for efficient phloem transport, are able to complete their life cycle and produce viable seed

Avinash C. Srivastava, Kasturi Dasgupta, Eric Ajieren, Gabriella Costilla, Roisin C. McGarry and Brian G. Ayre

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 104, issue 6, pages 1121-1128
Published in print November 2009 | ISSN: 0305-7364
Published online September 2009 | e-ISSN: 1095-8290 | DOI: http://dx.doi.org/10.1093/aob/mcp215
Arabidopsis plants harbouring a mutation in AtSUC2, encoding the predominant sucrose/proton symporter necessary for efficient phloem transport, are able to complete their life cycle and produce viable seed

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Background and Aims

AtSUC2 encodes a sucrose/proton symporter that localizes throughout the collection and transport phloem and is necessary for efficient transport of sucrose from source to sink tissues in Arabidopsis thaliana. Plants harbouring homozygous AtSUC2 null alleles accumulate sugar, starch, and anthocyanin in mature leaves, have severely delayed development and stunted growth and, in previous studies, failed to complete their life cycle by producing viable seed.

Methods

An AtSUC2 allele with a T-DNA insertion in the second intron was analysed. Full-length transcript from this allele is not produced, and a truncated protein translated from sequences upstream of the insertion site did not catalyse sucrose uptake into yeast, supporting the contention that this is a null allele. Mutant plants were grown in a growth chamber with a diurnal light/dark cycle, and growth patterns recorded.

Key Results

This allele (SALK_038124, designated AtSUC2-4) has the hallmarks of previously described null alleles but, despite compromised carbon partitioning and growth, produces viable seeds. The onset of flowering was chronologically delayed but occurred at the same point in the plastochron index as wild type.

Conclusions

AtSUC2 is important for phloem loading and is therefore fundamental to phloem transport and plant productivity, but plants can complete their life cycle and produce viable seed in its absence. Arabidopsis appears to have mechanisms for mobilizing reduced carbon from the phloem into developing seeds independent of AtSUC2.

Keywords: Arabidopsis thaliana; AtSUC2; sucrose; transporter; symporter; phloem; phloem loading; plant productivity

Journal Article.  5403 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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